Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus
Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus
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DOI：
10.1007/s00122-019-03469-z
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发表时间：
2019-11-05
影响因子：
5.4
通讯作者：
Walsh, John
中科院分区：
文献类型：
--
作者：
Hackenberg, Dieter;Asare-Bediako, Elvis;Walsh, John
关键信息在天然异源四倍体油菜品种Yudal中鉴定出与一个主要QTL相关的对萝卜黄病毒的部分显性抗性。摘要萝卜黄病毒（TuYV）由桃-马铃薯蚜虫（Myzus persicae）传播，并导致商业油菜作物（Brassica napus）的严重产量损失。目前，芸苔属植物中只有一种抗TuYV的遗传资源，它是在再合成的B中鉴定的。甘蓝型油菜品系R54。在我们的研究中，27例大多为纯合子B。甘蓝型油菜种质，双单倍体（DH）或自交系，代表B的不同子集。napus genepool进行TuYV抗性/感病性筛选。朝鲜春油菜B对TuYV有部分抗性。甘蓝型油菜品种Yudal，而矮生法国冬油菜品系Darmor-bzh是易感的。利用建立的Darmor-bzh × Yudal DH作图群体（DYDH）进行QTL定位，在两个独立的实验中发现一个主效QTL，分别解释了36%和18%的表型变异。将DYDH系与Yudal杂交，从F1与感病或抗病亲本的正反交回交（BC 1）群体揭示了TuYV抗性的显性遗传。在分离的BC 1群体中验证了ChrA 04上的QTL。在两个DYDH试验中的一个试验中鉴定了ChrC 05上的第二个微效QTL，在BC 1群体中未观察到。R54中的TuYV抗性QTL位于Yudal的Chr A04上的QTL区间内，但与R54共分离的标记在Yudal中不保守，表明TuYV抗性的独立起源。这是首次在天然B中进行TuYV抗性QTL定位的报道。油菜。
Key messagePartially dominant resistance to Turnip yellows virus associated with one major QTL was identified in the natural allotetraploid oilseed rape cultivar Yudal.AbstractTurnip yellows virus (TuYV) is transmitted by the peach-potato aphid (Myzus persicae) and causes severe yield losses in commercial oilseed rape crops (Brassica napus). There is currently only one genetic resource for resistance to TuYV available in brassica, which was identified in the re-synthesised B. napus line 'R54'. In our study, 27 mostly homozygous B. napus accessions, either doubled-haploid (DH) or inbred lines, representing a diverse subset of the B. napus genepool, were screened for TuYV resistance/susceptibility. Partial resistance to TuYV was identified in the Korean spring oilseed rape, B. napus variety Yudal, whilst the dwarf French winter oilseed rape line Darmor-bzh was susceptible. QTL mapping using the established Darmor-bzh x Yudal DH mapping population (DYDH) revealed one major QTL explaining 36% and 18% of the phenotypic variation in two independent experiments. A DYDH line was crossed to Yudal, and reciprocal backcross (BC1) populations from the F-1 with either the susceptible or resistant parent revealed the dominant inheritance of the TuYV resistance. The QTL on ChrA04 was verified in the segregating BC1 population. A second minor QTL on ChrC05 was identified in one of the two DYDH experiments, and it was not observed in the BC1 population. The TuYV resistance QTL in 'R54' is within the QTL interval on Chr A04 of Yudal; however, the markers co-segregating with the 'R54' resistance are not conserved in Yudal, suggesting an independent origin of the TuYV resistances. This is the first report of the QTL mapping of TuYV resistance in natural B. napus.