Single-channel activity of the Ca2+-dependent K+ channel is modulated by FK506 and rapamycin
Single-channel activity of the Ca2+-dependent K+ channel is modulated by FK506 and rapamycin
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DOI：
10.1016/s0006-8993(97)01435-2
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发表时间：
1998-03
期刊：
影响因子：
2.9
通讯作者：
A. Terashima;M. Nakai;T. Hashimoto;T. Kawamata;T. Taniguchi;M. Yasuda;K. Maeda;C. Tanaka
中科院分区：
文献类型：
--
作者：
A. Terashima;M. Nakai;T. Hashimoto;T. Kawamata;T. Taniguchi;M. Yasuda;K. Maeda;C. Tanaka
在原代培养的大鼠背侧海马神经元上进行单通道膜片钳记录。记录到神经元的Ca ~（2+）依赖性和TEA敏感性K ~+电流。应用免疫抑制剂FK 506和雷帕霉素的细胞膜内的通道显着延长通道的平均开放时间。钙调磷酸酶自抑制片段和W-7诱导的通道的平均开放时间没有显着的改变。这些结果表明，FK 506和雷帕霉素对Ca 2+依赖性K+通道活性的调节是直接通过免疫抑制剂与FKBP 12的结合实现的。
Single-channel patch clamp recordings were performed in primary cultured neurons from rat dorsal hippocampi. Ca2+-dependent and TEA-sensitive K+current was recorded from the neurons. Application of immunosuppressants FK506 and rapamycin to the channel inside the plasma membrane of the neurons significantly prolonged the mean open time of the channel. Calcineurin autoinhibitory fragment and W-7 induced no significant alteration in the mean open time of the channel. These results suggest that modulation of the activity of the Ca2+-dependent K+channel by FK506 and rapamycin is directly through association of immunosuppressants with FKBP12.