Change in PGC-1α Expression in Rat Skeletal Muscle after Low-intensity Prolonged Swimming Exercise
Change in PGC-1α Expression in Rat Skeletal Muscle after Low-intensity Prolonged Swimming Exercise
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DOI：
10.2114/jpa2.30.23
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Tabata, Izumi
中科院分区：
文献类型：
--
作者：
Fujimoto, Eri;Yamaguchi, Wataru;Tabata, Izumi
低强度游泳运动(LIE)后18h，大鼠骨骼肌整体和核组分中PGC-1α的含量均高于对照组。然而，没有研究报告在那之后PGC-1α含量发生了变化。因此，我们检测了大鼠骨骼肌中PGC-1α的整体和核组分，直到LIE后2411。此外，我们还研究了线粒体蛋白-氨基酮戊酸合成酶(ALAS)的mRNA的变化，以明确PGC-1α的哪一部分在运动后作为转录共激活因子具有增强线粒体氧化酶的生理作用。用免疫印迹法检测了6周龄雄性SD大鼠(体重180~200g)卧床后外周肌(EPI)中PGC-1α蛋白含量。用逆转录聚合酶链式反应(RT-PCR)定量检测ALAS的mRNA含量。大鼠EPI肌肉中PGC-1α的含量在卧床后18h和24小时分别比对照组高73%和75%。PGC-1α在肌肉和ALAS核组分中的含量分别比对照组高58%和25%，但在卧床后恢复到对照水平2411。结果表明，运动后外周肌肉核团中PGC-1α的含量与ALAS基因表达的时程一致，提示核内PGC-1α可能具有作为转录共激活因子的生理功能，促进运动后线粒体蛋白的表达。J Physiol Anthropol30(1)：23-27,2011年http://www.jstage.jst.go.jp/browse/jpa2[doi：10.2114/jpa2.30.23]
After 6-h low-intensity swimming exercise (LIE), peroxisome proliferator-activated receptor gamma coactivator -1 alpha (PGC-1 alpha) in whole and nuclear fractions in rat skeletal muscle was higher than the control rats' muscles up to 18 h after LIE. However, no study has reported change in PGC-1 alpha content after that. Therefore, we measured PGC-1 alpha in whole and nuclear fractions in rat skeletal muscle up to 2411 after LIE. Furthermore, we evaluated change in the mRNA of delta-aminolevulinate synthase (ALAS), a mitochondrial protein, to clarify in which fraction of PGC-1 alpha has a physiological role as a transcriptional coactivator for enhancing the mitochondrial oxidative enzymes after exercise. We measured PGC-1 alpha protein content in whole and nuclear fractions in the epitrochlearis (EPI) muscle of male Sprague-Dawley rats (age: 6w; body weight: 180-200g) after LIE by Western-blot analysis. The ALAS mRNA content was quantified by RTPCR. The PGC-1 alpha contents in whole fractions in the rat EPI muscle were 73% and 75% higher than that of the control rats' muscle, 18h and 24h after LIE, respectively. The PGC-1 alpha content in nuclear fractions in the muscle and ALAS mRNA was higher than that of the control rats' muscle by 58% and 25%, respectively, while they returned to the control level 2411 after LIE. The present investigation demonstrated that the time-course of PGC-1 alpha content in nuclear fractions in the EPI muscle was the same as the ALAS mRNA, suggesting that PGC-1 alpha in the nucleus may have a physiological function as a transcriptional coactivator for enhancing mitochondrial protein expression after exercise. J Physiol Anthropol 30(1): 23-27, 2011 http://www.jstage.jst.go.jp/browse/jpa2 [DOI: 10.2114/jpa2.30.23]