Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis
Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis
复制标题
DOI：
10.1007/s11130-011-0224-6
复制
发表时间：
2011-06-01
影响因子：
4
通讯作者：
Qiu, Hong-Wei
中科院分区：
文献类型：
--
作者：
Xiao, Jun-Xia;Huang, Guo-Qing;Qiu, Hong-Wei
异黄酮是在各种植物源性食品中发现的主要生物活性化合物之一，特别是在大豆和大豆食品中。本文研究了大豆提取物混合物（SI-Ⅰ，含大豆苷元71%、染料木素14.3%、黄豆黄素14.7%）对HeLa细胞的作用及其机制。MTT法显示5-80 μ g/ml浓度的SI-I对HeLa细胞的存活率有明显的抑制作用，而对L929细胞无明显的毒副作用。用10、20和40 μ g/ml的SI-I处理HeLa细胞4d后，荧光显微镜和激光共聚焦扫描显微镜分别观察到细胞核碎裂、胞浆皱缩和体积缩小等典型的凋亡形态学改变。流式细胞仪分析显示，与对照组相比，Deltapsi m丢失的早期凋亡细胞百分比分别增加了2.27、2.74和4.05倍。结果表明，SI-I通过线粒体途径诱导HeLa细胞凋亡，抑制HeLa细胞生长，并与文献报道的结果进行了比较，结果表明SI-I中所含的多种生物活性物质之间存在协同作用。因此，大豆黄酮类化合物是一种潜在的抗宫颈癌的化学治疗药物。
Isoflavones are among the major bioactive compounds found in a wide variety of plant-derived foods, especially in soybeans and soy-based foods. In this study, the effect of a soy-derived isoflavone mixture (designated as SI-I, containing 71% daidzein, 14.3% genistein and 14.7% glycitein) on HeLa cells and its mechanism were investigated. SI-I in concentration range 5-80 mu g/ml significantly reduced the survival rate of HeLa cells by MTT assay, whereas showed no side effect on that of L929 cells. After HeLa cells were exposed to 10, 20 and 40 mu g/ml SI-I for 4 days, typical apoptotic morphological changes, including nuclear fragmentation, cytoplasm shrinkage and decrease of cell volume, were observed by fluorescence microscope and CLSM, respectively. FCM analysis revealed that the percentages of early apoptotic cells with lost Delta psi m increased by 2.27, 2.74 and 4.05 folds respectively, compared with control. The results showed that SI-I inhibited HeLa cell growth through inducing apoptosis via the mitochondrial pathway and comparisons with reported data indicated that synergistic effect existed between the isoflavone species contained in SI-I. It is proposed that natural soy-derived isoflavones are potential candidates as chemoterapeutic agents against human cervical cancer.