Efficient Preparation of Streptochlorin from Marine Streptomyces sp. SYYLWHS-1-4 by Combination of Response Surface Methodology and High-Speed Counter-Current Chromatography.
Efficient Preparation of Streptochlorin from Marine Streptomyces sp. SYYLWHS-1-4 by Combination of Response Surface Methodology and High-Speed Counter-Current Chromatography.
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DOI：
10.3390/molecules21060693
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发表时间：
2016-05-27
期刊：
影响因子：
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通讯作者：
Yan X
中科院分区：
文献类型：
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作者：
Li L;He S;Ding L;Yuan Y;Zhu P;Epstein S;Fan J;Wu X;Yan X
链氯素是从链霉菌SF 2583的亲脂性提取物中首次分离得到的一种具有抗菌、抗过敏、抗肿瘤、抗炎等多种药理活性的化合物。为了从链霉菌SYYLWHS-1-4中高效制备链氯素，建立了响应面法（RSM）和高速逆流色谱（HSCCC）相结合的方法。在发酵过程中，我们利用响应面法优化链氯素高效积累的条件，最佳参数为：酵母膏1.889 g/L，可溶性淀粉8.636 g/L，K2HPO40.359 g/L，CaCl22.5 g/L，MgSO40.625 g/L，海盐25 g/L，培养基体积50%，初始pH值7.0，温度27.5 °C，这将链氯素产率提高了17.7倍。在纯化过程中，使用石油醚-乙酸乙酯-甲醇-水（9：0.8：5：5，v/v/v/v）两相溶剂系统进行制备性HSCCC分离，其中300 mg粗样品得到16.5mg链氯素，通过UPLC测定纯度超过95%。因此，该组合方法为高效制备链氯素提供了可行的策略，从而确保了大量链氯素的供应，用于体内药理学评估或其他需求。
Since first isolated from the lipophilic extract of Streptomyces sp. SF2583, streptochlorin, has attracted a lot of attention because of its various pharmacological properties, such as antibiotic, antiallergic, antitumor, and anti-inflammatory activities. For the efficient preparation of streptochlorin from a producing strain Streptomyces sp. SYYLWHS-1-4, we developed a combinative method by using response surface methodology (RSM) and high-speed counter-current chromatography (HSCCC). In the fermentation process, we used RSM to optimize the condition for the efficient accumulation of streptochlorin, and the optimal parameters were: yeast extract 1.889 g/L, soluble starch 8.636 g/L, K2HPO4 0.359 g/L, CaCl2 2.5 g/L, MgSO4 0.625 g/L, marine salt 25 g/L, medium volume 50%, initial pH value 7.0, temperature 27.5 °C, which enhanced streptochlorin yield by 17.7-fold. During the purification process, the preparative HSCCC separation was performed using a petroleum ether–ethyl acetate–methanol–water (9:0.8:5:5, v/v/v/v) biphasic solvent system, where 300 mg of crude sample yielded 16.5 mg streptochlorin with over 95% purity as determined by UPLC. Consequently, the combination method provided a feasible strategy for highly effective preparation of streptochlorin, which ensured the supply of large amounts of streptochlorin for in vivo pharmacological assessments or other requirements.