Effect of Interleukin-6 (IL-6) on the Vascular Smooth Muscle Contraction in Abdominal Aorta of Rats with Streptozotocin-Induced Diabetes
Effect of Interleukin-6 (IL-6) on the Vascular Smooth Muscle Contraction in Abdominal Aorta of Rats with Streptozotocin-Induced Diabetes
复制标题
白介素6（IL-6）对链脲佐菌素糖尿病大鼠腹主动脉血管平滑肌收缩的影响
DOI：
10.4077/cjp.2011.amm069
复制
发表时间：
2011-10-31
影响因子：
1.8
通讯作者：
Xie, Dong-Ping
中科院分区：
文献类型：
--
作者：
Tang, Wen-Bo;Zhou, Yu-Qin;Xie, Dong-Ping
Patients with type 1 diabetes are at a risk of hypertension. However, the mechanisms behind the findings are not completely known. The aim of the present study was to investigate involvement of interleukin-6 (IL-6) on the contraction of abdominal aorta in rats with type 1 diabetes. IL-6 levels in the plasma of rats with streptozotocin (STZ)-induced diabetes were determined by ELISA. The abdominal aorta was dissected free of fat and connective tissues and then cut into spiral rings. The endothelium-denuded strip was vertically suspended in tissue chambers containing 5 ml Krebs solution at 37 C and bubbled continuously with 95% O-2-5% CO2. The effects of phenylephrine (Phe) on the contractile responses of abdominal aorta were recorded. The effects of IL-6 and anti-rat IL-6 antibody on the Phe-induced response were also examined. Plasma levels of IL-6 increased time-dependently in rats with STZ-induced diabetes. Phe caused concentration-dependent contraction in aortic rings. Phe-induced contractions were higher in vascular strips of STZ-induced diabetic rats than that of control rats. Pretreatment of vascular strips with IL-6 for 1 h did not cause contraction but enhanced the contraction in response to Phe. Treatment of the vascular strips with an anti-IL-6 antibody for 1 h decreased the Phe-induced contractions. These results suggest that IL-6 causes vascular smooth muscle contraction in abdominal aorta of rats with type 1 diabetes.
