All IncP-1 plasmid subgroups, including the novel ε subgroup, are prevalent in the influent of a Danish wastewater treatment plant
All IncP-1 plasmid subgroups, including the novel ε subgroup, are prevalent in the influent of a Danish wastewater treatment plant
复制标题
DOI：
10.1016/j.plasmid.2009.05.004
复制
发表时间：
2009-09-01
期刊：
影响因子：
2.6
通讯作者：
Sorensen, Soren J.
中科院分区：
文献类型：
--
作者：
Bahl, Martin Iain;Burmolle, Mette;Sorensen, Soren J.
IncP-1质粒的存在和多样性的丹麦污水处理厂的进水进行了研究，通过PCR扩增的trfA基因的社区DNA测序。设计了三套PCR引物，从所有目前测序的IncP-1质粒中扩增出281 bp的trfA片段。基于72个获得的序列与先前公布的IncP-1质粒的同源序列的多重比对的邻接树揭示了IncP-1质粒的所有已建立的亚组，α，β，γ和δ，都存在于废水处理厂流入物中。还在废水中检测到代表最近描述的第五亚组，即第五亚组的序列。因此，这些结果证实了IncP-1质粒的至少五个遗传学上不同的亚组的存在，并代表了首次在单一环境中检测到与所有这五个亚组的质粒相关的序列。此外，结果证实，废水构成了接合IncP-1质粒的储存库，这些质粒通常含有多种抗生素抗性基因。(c)2009 Elsevier Inc. All rights reserved.
The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, alpha, beta, gamma and delta, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the epsilon subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes. (c) 2009 Elsevier Inc. All rights reserved.