Human Proteome Project Mass Spectrometry Data Interpretation Guidelines 2.1.
Human Proteome Project Mass Spectrometry Data Interpretation Guidelines 2.1.
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人蛋白质组项目质谱数据解释指南2.1。
DOI：
10.1021/acs.jproteome.6b00392
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发表时间：
2016-11-04
影响因子：
4.4
通讯作者：
Omenn GS
中科院分区：
文献类型：
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作者：
Deutsch EW;Overall CM;Van Eyk JE;Baker MS;Paik YK;Weintraub ST;Lane L;Martens L;Vandenbrouck Y;Kusebauch U;Hancock WS;Hermjakob H;Aebersold R;Moritz RL;Omenn GS
每一项数据丰富的社区研究工作都需要一个明确的计划，以确保数据解释的质量和分析的可比性。为了满足人类蛋白质组组织（HUPO）的人类蛋白质组计划（HPP）的这一需求，我们通过广泛的咨询制定了一套质谱数据解释指南，应适用于所有HPP数据贡献。对于报告HPP蛋白鉴定结果的手稿的提交，指南以一页清单的形式呈现，其中包含15个要点，然后是每个要点的两页扩展描述。在这里，我们对指南进行了概述，并对15个要素中的每一个进行了深入描述，以便于作者和评审人员理解指南背后的意图和原理。从广义上讲，这些指南提供了关于如何将HPP数据提交给质谱数据库，如何进行错误分析以及如何用额外的确证性证据支持新蛋白质的检测的具体指导。这些由HPP社区制定的指南将提交给更广泛的科学界进行进一步讨论。
Every data-rich community research effort requires a clear plan for ensuring the quality of the data interpretation and comparability of analyses. To address this need within the Human Proteome Project (HPP) of the Human Proteome Organization (HUPO), we have developed through broad consultation a set of mass spectrometry data interpretation guidelines that should be applied to all HPP data contributions. For submission of manuscripts reporting HPP protein identification results, the guidelines are presented as a one-page checklist containing fifteen essential points followed by two pages of expanded description of each. Here, we present an overview of the guidelines and provide an in-depth description of each of the fifteen elements to facilitate understanding of the intentions and rationale behind the guidelines, both for authors and for reviewers. Broadly, these guidelines provide specific directions regarding how HPP data are to be submitted to mass spectrometry data repositories, how error analysis should be presented, and how detection of novel proteins should be supported with additional confirmatory evidence. These guidelines, developed by the HPP community, are presented to the broader scientific community for further discussion.