A competitive immunochromatographic assay based on a novel probe for the detection of mercury (II) ions in water samples
A competitive immunochromatographic assay based on a novel probe for the detection of mercury (II) ions in water samples
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基于新型探针的竞争性免疫色谱法检测水样中的汞 (II) 离子
DOI：
10.1016/j.bios.2010.04.003
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发表时间：
2010-07-15
影响因子：
12.6
通讯作者：
Liu, Zengshan
中科院分区：
文献类型：
--
作者：
Zhou, Yu;Zhang, Yuanyuan;Liu, Zengshan
汞离子（Hg2+）是最危险的污染物之一。即使浓度很低，也会造成严重的环境和健康问题。目前环境样品中Hg2+的检测方法繁琐且耗时，因为它们需要复杂的仪器和复杂的样品前处理过程。本文合成了一种对Hg2+具有高选择性的新型探针，并在此基础上建立了一种用于水样中Hg2+检测的一步竞争性免疫层析分析方法。将检测偶联物固定在硝化纤维素膜的一端（检测线），将抗bsa多克隆抗体固定在膜的另一端（控制线）。样品中的Hg2+与探针竞争，与固定化检测偶联物结合。加标水样中Hg2+的视觉检出限约为1 ppb。定性分析可在15分钟内完成。该技术的优点是快速，成本低，不需要任何设备和复杂的样品制备。(C) 2010 Elsevier B.V.版权所有
Mercury ions (Hg2+) are one of the most dangerous pollutants. Even at low concentration, it causes serious environmental and health problems. Current methods for the detection of Hg2+ in environmental samples are tedious and time consuming because they require sophisticated instrumentation and complicated sample pre-treatment processes. In this work, a novel probe with high selectivity towards Hg2+ was synthesized and a one step competitive immunochromatographic assay based on the probe for the detection of Hg2+ was developed and applied for water samples. The detection conjugate was immobilized on one end of the nitrocellulose membrane (detection line) and anti-BSA polyclonal antibody was immobilized on the other end of the membrane (control line). Hg2+ in samples competed with the probe to bind with immobilized detection conjugate. The visual detection limit of Hg2+ in spiked water samples was found to be about 1 ppb. The qualitative assay can be performed within 15 min. The advantages of the technique are rapidity, low cost and without the need of any equipment and complicated sample preparation. (C) 2010 Elsevier B.V. All rights reserved.