Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data.
Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data.
复制标题
QualiMap 2：高通量测序数据的高级多样本质量控制。
DOI：
10.1093/bioinformatics/btv566
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发表时间：
2016-01-15
期刊：
影响因子：
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通讯作者：
García-Alcalde F
中科院分区：
文献类型：
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作者：
Okonechnikov K;Conesa A;García-Alcalde F
动机：检测随机误差和系统偏差是处理高通量测序（HTS）数据的强大管道的关键步骤。能够执行这项任务的生物信息学软件工具是可用的，无论是用于HTS数据的一般分析还是针对特定测序技术。然而，大多数现有的QC仪器一次只能处理一个样品。结果：Qualimap 2代表了HTS数据QC分析的下一步。除了对校准数据进行全面的单样本分析外，它还包括允许同时处理和比较多个样本的新模式。与第一个版本一样，新特性可以通过图形和命令行界面使用。此外，它还包括用户社区提出的大量改进。可用性和实现：该软件的实现以及文档可以在http://www.qualimap.org上免费获得。补充信息：补充数据可在Bioinformatics在线获取。
Motivation: Detection of random errors and systematic biases is a crucial step of a robust pipeline for processing high-throughput sequencing (HTS) data. Bioinformatics software tools capable of performing this task are available, either for general analysis of HTS data or targeted to a specific sequencing technology. However, most of the existing QC instruments only allow processing of one sample at a time. Results: Qualimap 2 represents a next step in the QC analysis of HTS data. Along with comprehensive single-sample analysis of alignment data, it includes new modes that allow simultaneous processing and comparison of multiple samples. As with the first version, the new features are available via both graphical and command line interface. Additionally, it includes a large number of improvements proposed by the user community. Availability and implementation: The implementation of the software along with documentation is freely available at http://www.qualimap.org. Contact: meyer@mpiib-berlin.mpg.de Supplementary information: Supplementary data are available at Bioinformatics online.