Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology.
Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology.
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DOI：
10.1615/critrevbiomedeng.2020033450
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发表时间：
2020
影响因子：
--
通讯作者：
Taylor RE
中科院分区：
文献类型：
--
作者：
Beltrán SM;Slepian MJ;Taylor RE
在纳米尺度上，推力、拉力和剪切力驱动发育和重塑以及伤口愈合和疾病进展中的生化过程。机械生物学领域的研究不仅研究这些负荷如何影响生化信号通路，而且还研究信号通路如何通过触发机械变化（如组织的区域硬化）来响应局部负荷。机械和生物化学信号之间的这种反馈越来越被认为是胚胎发育、组织形态发生、细胞信号和疾病发病机制的基础。从历史上看，机械生物学的跨学科领域一直受到测量和操纵细胞和分子力的技术发展的推动，每一种新工具都能带来广阔的新研究领域。在这篇综述中，我们讨论了分子尺度力和应变传感器的制造和能力的最新进展。我们还展示了DNA纳米技术如何对增强现有技术和开发未来机械传感器组装的独特能力至关重要。DNA是用于传感器制造的响应性和可编程的构建材料。它能够系统地询问分子生物力学，其力为1至200 pN，这是阐明细胞和蛋白质传递机械信号的基本手段所需的。
At the nanoscale, pushing, pulling, and shearing forces drive biochemical processes in development and remodeling as well as in wound healing and disease progression. Research in the field of mechanobiology investigates not only how these loads affect biochemical signaling pathways but also how signaling pathways respond to local loading by triggering mechanical changes such as regional stiffening of a tissue. This feedback between mechanical and biochemical signaling is increasingly recognized as fundamental in embryonic development, tissue morphogenesis, cell signaling, and disease pathogenesis. Historically, the interdisciplinary field of mechanobiology has been driven by the development of technologies for measuring and manipulating cellular and molecular forces, with each new tool enabling vast new lines of inquiry. In this review, we discuss recent advances in the manufacturing and capabilities of molecular-scale force and strain sensors. We also demonstrate how DNA nanotechnology has been critical to the enhancement of existing techniques and to the development of unique capabilities for future mechanosensor assembly. DNA is a responsive and programmable building material for sensor fabrication. It enables the systematic interrogation of molecular biomechanics with forces at the 1- to 200-pN scale that are needed to elucidate the fundamental means by which cells and proteins transduce mechanical signals.