Regional heterogeneity of muscarinic acetylcholine receptors from rat brain is retained after detergent solubilization.
Regional heterogeneity of muscarinic acetylcholine receptors from rat brain is retained after detergent solubilization.
复制标题
去污剂溶解后，大鼠脑中毒蕈碱乙酰胆碱受体的区域异质性得以保留。
DOI：
10.1016/s0304-3940(85)80119-1
复制
发表时间：
1985
影响因子：
2.5
通讯作者：
Aronstam,RS
中科院分区：
文献类型：
--
作者：
Wenger,DA;Parthasarthy,N;Aronstam,RS
Muscarinic acetylcholine receptors were extracted from rat cerebral cortex, hippocampus and brainstem membranes with 1% digitonin. Solubilized receptors retained both multiple receptor affinities and their regional selectivity in agonist binding (in terms of their affinity for carbamylcholine, brainstem>cortex>hippocampus). The affinity for carbamylcholine was increased 2–8-fold by solubilization; this increase was largely accounted for by an increase in the proportion of receptors displaying high affinity binding. No differences were observed in the size of receptor-detergent complexes from cortex or brainstem in gel filtration chromatography using a variety of gels and solubilizing agents. These findings indicate that not all of the factors underlying receptor heterogeneity and conformational state are removed or disrupted by solubilization.
