Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora, mucin histochemical characterization, and immune populations of weanling pigs
Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora, mucin histochemical characterization, and immune populations of weanling pigs
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DOI：
10.1016/j.livsci.2007.01.063
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发表时间：
2007-05-01
影响因子：
1.8
通讯作者：
Rehberger, T.
中科院分区：
文献类型：
--
作者：
Davis, M. E.;Brown, D. C.;Rehberger, T.
直接喂食微生物（DFM）能够改变胃肠道微生物菌群、形态和免疫力。采用变性梯度凝胶电泳分析短乳杆菌、芽孢杆菌和抗生素补充对胃肠道微生物群的影响。处理以2 × 3析因排列，具有两个水平的L。brevis补充和三种饮食（对照，芽孢杆菌和抗生素）喂养在保育期。胃肠道微生物群的微生物多样性增加，以响应任何含有L。短杆菌、芽孢杆菌或抗生素。猪提供L.短杆菌和芽孢杆菌联合处理的猪比单独抗生素处理的猪表现出更多的高G/C含量条带。补充L. brevis产生了一条独特的条带，该条带被鉴定为不可培养的、低G/C含量的革兰氏阳性细菌。此外，L.与未提供L. brevis的猪相比，在保育期结束时，猪体重增加了1.6 kg。brevis。L.短芽孢杆菌可增加十二指肠和空肠绒毛上酸性杯状细胞的数量，并减少硫酸化杯状细胞的数量，而芽孢杆菌和抗生素补充可减少十二指肠绒毛上硫酸化杯状细胞的数量。免疫组化结果显示L.空肠绒毛内CD 2（+）细胞、抗原呈递细胞（MHCII+）和CD 4（+）T细胞的数量降低。这些数据说明了DFM提高断奶后猪生长速度的潜力，以及引起肠道微生物群落、产生粘蛋白的杯状细胞和免疫细胞的改变。(c)2007 Elsevier B. V.保留所有权利。
Direct-fed microbials (DFM) have the ability to alter gastrointestinal microflora, morphology and immunity. Denaturing gradient gel electrophoresis was used to analyze the effect of Lactobacillus brevis, Bacillus, and antibiotic supplementation on the gastrointestinal microbiota. Treatments were arranged in a 2 x 3 factorial arrangement with two levels of L. brevis supplementation and three diets (control, Bacillus, and antibiotic) fed during the nursery period. Microbial diversity of the gastrointestinal microbiota increased in response to any of the treatments containing L. brevis, Bacillus, or antibiotic compared to unsupplemented pigs. Pigs provided L. brevis and Bacillus in combination exhibited more bands of high G/C content than pigs provided only the antibiotic treatment. Supplementation with L. brevis resulted in a unique band that was identified as unculturable, low G/C content, gram positive bacteria. Furthermore, L. brevis increased pig body weight at the end of the nursery by 1.6 kg compared to pigs not provided L. brevis. L. brevis increased the number of acidic goblet cells present on villi within the duodenum and jejunum, and decreased the number of sulfated goblet cells, whereas Bacillus and antibiotic supplementation decreased the number of sulfated goblet cells on villi within the duodenum. Immunohistochemical evaluation revealed that L. brevis supplementation lowered the number of CD2(+) cells, antigen presenting cells (MHCII+), and CD4(+) T cells within jejunal villi. These data illustrate the potential for DFM to improve pig growth rate after weaning, as well as elicit alterations in the gut microbial community, mucin-producing goblet cells, and immune cells. (c) 2007 Elsevier B.V. All rights reserved.