Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland
Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland
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DOI：
10.1099/ijsem.0.003964
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发表时间：
2020-01-01
影响因子：
2.8
通讯作者：
Tian, Yongqiang
中科院分区：
文献类型：
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作者：
Hu, Mengyao;Li, Xiaoguang;Tian, Yongqiang
从成都市双流区农田采集的枕油葫芦（Teleogryllus occipitalis）中分离到一株革兰氏染色阴性、不产芽孢、能运动、需氧、杆状的细菌，命名为LCB 8（T）。根据16 S rRNA基因序列进行系统发育分析，该菌株属于布鲁氏菌科α变形菌纲苍白杆菌属（Ochrobactrum）。猪苍白杆菌CCUG 60088（T）（97.9%）和嗜血苍白杆菌CCUG 38531（T）（98.8%）与LCB 8（T）的亲缘关系最近。该新菌株能够在0-4.5%（w/v）的盐浓度、pH 5-9和20-42 ℃的温度下生长。主要的醌系统是泛醌0-10，主要的脂肪酸是C-18：1 ω 7 c、C-16（：0）和C-18：0。主要极性脂质为磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰单甲基乙醇胺、二磷脂酰甘油和4种未定义的氨基脂质。腐胺和亚精胺是主要的多胺。基因组测序显示基因组大小为4.76 Mbp，DNA G+C含量为57.1 mol%。这些表型、基因型和化学分类学特征很好地支持了LCB 8（T）与苍白杆菌属的关系。成对测定的全基因组平均核苷酸同一性（ANI）值表明菌株LCB 8（T）代表一个新物种，我们建议将其命名为Teleogrylli苍白杆菌新种（Ochrobactrum teleogrylli sp. nov.），模式菌株LCB 8（T）（=KCTC 72031（T）=CGMCC 1.13984（T））。
A Gram-stain-negative, non-spore-forming, motile, aerobic, rod-shaped bacteria strain, designated LCB8(T), was isolated from the insect Teleogryllus occipitalis captured from a deserted cropland in Shuangliu district, Chengdu, PR China. Phylogenetic analysis on the basis of 16S rRNA gene sequence indicated that the strain represented a member of the genus Ochrobactrum, family Brucellaceae, class Alphaproteobacteria. Ochrobactrum pecoris CCUG 60088(T) (97.9%) and Ochrobactrum haematophilum CCUG 38531(T) (98.8%) were identified as the most closely related phylogenetic neighbours of strain LCB8(T). The novel strain was able to grow at salt concentrations of 0-4.5% (w/v), pH 5-9 and temperatures of 20-42 degrees C. The major quinone system was ubiquinone 0-10, the major fatty acids were C-18:1 omega 7c, C-16(:0) and C-18:0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and four undefined aminolipids. The major polyamines were putrescine and spermidine. Genome sequencing revealed a genome size of 4.76 Mbp and a DNA G+C content of 57.1 mol%. These phenotypic, genotypic and chemotaxonomic traits excellently supported the affiliation of LCB8(T) to the genus Ochrobactrum. Pairwise determined whole-genome average nucleotide identity (ANI) values indicated that strain LCB8(T) represents a novel species, for which we propose the name Ochrobactrum teleogrylli sp. nov. with the type strain LCB8(T) (=KCTC 72031(T)=CGMCC 1.13984(T)).