OVEREXPRESSION OF CADHERINS AND UNDEREXPRESSION OF BETA-CATENIN INHIBIT DORSAL MESODERM INDUCTION IN EARLY XENOPUS EMBRYOS
OVEREXPRESSION OF CADHERINS AND UNDEREXPRESSION OF BETA-CATENIN INHIBIT DORSAL MESODERM INDUCTION IN EARLY XENOPUS EMBRYOS
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DOI：
10.1016/0092-8674(94)90069-8
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发表时间：
1994-12-02
期刊：
影响因子：
64.5
通讯作者：
WYLIE, C
中科院分区：
文献类型：
--
作者：
HEASMAN, J;CRAWFORD, A;WYLIE, C
钙黏蛋白 - 连环蛋白复合物在细胞 - 细胞黏附中具有重要作用，并且可能也在信号通路中发挥功能。我们报道在非洲爪蟾胚胎中三种钙黏蛋白类型的过度表达导致它们发育出的背轴结构减少。在通过与β - 连环蛋白mRNA互补的反义寡脱氧核苷酸去除母体β - 连环蛋白的胚胎中产生了相同的表型。它们显示出背中胚层标志物MyoD和 Goosecoid的表达受到抑制，但腹侧和一般中胚层标志物的表达未受抑制。它们缺乏脊索、体节和神经管，并且在Nieuwkoop试验中背中胚层信号传导存在缺陷。这种表型可以通过注射β - 连环蛋白mRNA而不是通过注射Xwnt - 8 mRNA来挽救。这些结果表明β - 连环蛋白在背中胚层诱导中具有重要作用。它们直接证明了母体mRNA在轴特化中的活性。
The cadherin-catenin complex has an important role in cell-cell adhesion and may also function in signaling pathways. We report that overexpression of three cadherin types in Xenopus embryos causes them to develop with reduced dorsal axial structures. The same phenotype is produced in embryos that have been depleted of maternal beta-catenin protein by an antisense oligodeoxynucleotide complementary to beta-catenin mRNA. They show an inhibition in the expression of dorsal mesodermal markers MyoD and goosecoid, but not of ventral and general mesodermal markers. They lack notochords, somites, and neural tubes and are defective in dorsal mesodermal signaling in Nieuwkoop assays. The phenotype can be rescued by the injection of beta-catenin mRNA and not by the injection of Xwnt-8 mRNA. These results show that beta-catenin has an important role in dorsal mesoderm induction. They directly demonstrate the activity of a maternal mRNA in axis specification.