Controlled Intracellular Release of Peptides from Microcapsules Enhances Antigen Presentation on MHC Class I Molecules
Controlled Intracellular Release of Peptides from Microcapsules Enhances Antigen Presentation on MHC Class I Molecules
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DOI：
10.1002/smll.200900809
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发表时间：
2009-10-02
期刊：
影响因子：
13.3
通讯作者：
Springer, Sebastian
中科院分区：
文献类型：
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作者：
Palankar, Raghavendra;Skirtach, Andre G.;Springer, Sebastian
为了了解单个细胞内任何小分子的作用时间过程，人们将在细胞内存款一定量，并在特定时刻启动其活动。实现这一目标的一种优雅方法是将分子封装在一个微米大小的容器中，将其引入细胞，通过激光脉冲远程打开其壁，然后通过显微镜跟踪生物反应。这种方法的有效性在这里使用具有限定壁的微胶囊进行验证，所述限定壁掺杂有金属纳米颗粒，以便使它们能够用红外激光打开。胶囊装载有荧光抗原肽，并引入哺乳动物培养细胞中，在激光诱导释放后，该肽与主要组织相容性复合体（MHC）I类蛋白结合，并增强其细胞表面转运。在细胞内释放药物并遵循其作用的概念适用于细胞生物学和医学中的许多问题。
To understand the time course of action of any small molecule inside a single cell, one would deposit a defined amount inside the cell and initiate its activity at a defined moment. An elegant way to achieve this is to encapsulate the molecule in a micrometer-sized reservoir, introduce it into a cell, remotely open its wall by a laser pulse, and then follow the biological response by microscopy. The validity of this approach is validated here using microcapsules with defined walls that are doped with metallic nanoparticles so as to enable them to be opened with an infrared laser. The capsules are loaded with a fluorescent antigenic peptide and introduced into mammalian cultured cells where, upon laser-induced release, the peptide binds to major histocompatibility complex (MHC) class I proteins and elicits their cell surface transport. The concept of releasing a drug inside a cell and following its action is applicable to many problems in cell biology and medicine.