DMACM-caged adenosine nucleotides: Ultrafast phototriggers for ATP, ADR and AMP activated by long-wavelength irradiation
DMACM-caged adenosine nucleotides: Ultrafast phototriggers for ATP, ADR and AMP activated by long-wavelength irradiation
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DOI：
10.1002/cbic.200390027
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发表时间：
2003-03-03
期刊：
影响因子：
3.2
通讯作者：
Hagen, V
中科院分区：
文献类型：
--
作者：
Geissler, D;Kresse, W;Hagen, V
本文介绍了基于[7-（二甲氨基）香豆素-4-基]甲基（DMACM）酯的光化学反应的新型光可切割腺苷核苷酸的开发。光触发剂在334 ~ 405 nm的紫外/可见照射下释放三磷酸腺苷（ATP）、二磷酸腺苷和单磷酸腺苷。由于适当的量子产率和高吸收率的结合，在长波长的光解理效率很高。利用时间分辨荧光光谱法测定了dmacm笼中ATP释放速率常数的下限为1.6x10(9) s（-1）。共聚焦激光扫描显微镜在生理研究中证实了dmacm笼化ATP的良好特性。我们能够在小鼠星形胶质细胞培养物和小鼠脑组织切片中释放dmacm笼的ATP，并能够测量光释放ATP对星形胶质细胞细胞反应的影响，即ATP引起CA（2+）离子波的能力。
The development of new photocleavable adenosine nucleotides based on the photochemistry of [7-(dimethylamino)coumarin-4-yl]methyl (DMACM) esters is described. The phototriggers liberate adenosine triphosphate (ATP), diphosphate, and monophosphate upon UV/Vis irradiation between 334 and 405 nm. The efficiency of photocleavage at long wavelengths is high as a result of a combination of appropriate quantum yields and intensive absorptivities. By using time-resolved fluorescence spectroscopy, we determined a lower limit of 1.6x10(9) s(-1) for the rate constant of the release of ATP from DMACM-caged ATP. The favorable properties of DMACM-caged ATP were confirmed in physiological studies by confocal laser scanning microscopy. We were able to uncage DMACM-caged ATP in cultures of mouse astrocytes and in brain tissue slices from mice and were also able to measure the effect of photoreleased ATP on the cellular response of astrocytes, namely the ability of the ATP to evoke CA(2+) ion waves.