Hydrogen-Bonded Biohybrid Framework-Derived Highly Specific Nanozymes for Biomarker Sensing.
Hydrogen-Bonded Biohybrid Framework-Derived Highly Specific Nanozymes for Biomarker Sensing.
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DOI：
10.1021/acs.analchem.1c03381
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发表时间：
2021-10
影响因子：
7.4
通讯作者：
Niru Ye;Siming Huang;Huangsheng Yang;Tong Wu;Linjing Tong;Fang Zhu;Guosheng Chen;Gangfeng Ouyang
中科院分区：
文献类型：
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作者：
Niru Ye;Siming Huang;Huangsheng Yang;Tong Wu;Linjing Tong;Fang Zhu;Guosheng Chen;Gangfeng Ouyang
纳米酶由于其在生物医学传感和免疫测定中有利的酶模拟活性和高稳定性而特别令人感兴趣。在这项工作中，我们报告了一个高度特异性的N-掺杂纳米酶通过热解框架限制牛血清白蛋白（BSA）。这种策略允许人们将低成本和无特征的BSA翻译成高活性的酶模拟物。所获得的碳纳米酶（表示为HBF-1-C800）与常规的碳纳米酶相比显示出3至7倍的过氧化物酶（POD）活性增强，并且还显示出约100%的酶活性。5-与所报道的N-掺杂石墨烯相比，其活性增强倍数。这种优异的POD活性源于高的N-掺杂效率，蛋白质诱导的缺陷位点，以及HBF-1-C800的固有多孔结构，其提供了丰富的可接近的活性位点，同时加速底物扩散。重要的是，HBF-1-C800纳米酶具有高度特异性的POD活性，并且还能够抵抗几种会使天然酶变性的苛刻条件。这些特性使其具有高精度，稳定性和灵敏度，适用于生物传感应用。此外，HBF-1-C800已被设计为一个有前途的平台，用于多种生物标志物的比色生物传感，包括H2 O2，谷胱甘肽和葡萄糖，具有宽的线性范围和低检测限，满足疾病诊断。
Nanozymes are of particular interest due to their enzyme-mimicking activity and high stability that are favorable in biomedical sensing and immunoassays. In this work, we report a highly specific N-doped nanozyme through pyrolysis of framework-confined bovine serum albumin (BSA). This strategy allows one to translate the low-cost and featureless BSA into a highly active enzyme mimic. The obtained carbon nanozyme (denoted as HBF-1-C800) displays 3- to 7-fold enhancement on peroxidase (POD) activity compared with the conventional carbon nanozymes and also shows ca. 5-fold activity enhancement compared to the reported N-doping graphene. Such excellent POD activity originates from high N-doping efficiency, protein-induced defective sites, and the intrinsic porous structure of HBF-1-C800, which provides abundantly accessible active sites and accelerates substrate diffusion simultaneously. Importantly, the HBF-1-C800 nanozyme has highly specific POD activity and also enables resistance to several harsh conditions that should denature natural enzymes. These features allow it with high accuracy, stability, and sensitivity for biosensing applications. Moreover, HBF-1-C800 has been designed as a promising platform for colorimetric biosensing of several biomarkers including H2O2, glutathione, and glucose, with wide linear ranges and low limits of detection that are satisfied with the disease diagnosis.