Frequency of Chlamydia trachomatis-specific T cell interferon-γ and interleukin-17 responses in CD4-enriched peripheral blood mononuclear cells of sexually active adolescent females
Frequency of Chlamydia trachomatis-specific T cell interferon-γ and interleukin-17 responses in CD4-enriched peripheral blood mononuclear cells of sexually active adolescent females
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DOI：
10.1016/j.jri.2014.01.002
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发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Darville, Toni
中科院分区：
文献类型：
--
作者：
Barral, Romina;Desai, Ruchi;Darville, Toni
在自然感染暴露的青少年女性队列中进行了对候选沙眼衣原体疫苗抗原的CD 4 T细胞应答的评价，以探索抗原免疫原性及其与预防再感染的相关性。通过ELISPOT测定外周血CD 4 T细胞IFN-γ和IL-17对三种候选疫苗抗原多态性膜蛋白G（PmpG）、F（PmpF）和主要外膜蛋白（MOMP）的应答频率;包括对衣原体热休克蛋白60（HSP 60）和单质体（EB）的应答以进行比较。比较了感染者（n = 8）、血清阳性/未感染者（n = 13）和血清阴性/未感染者（n = 18）的反应。与血清阴性/未感染的女性受试者相比，感染（P = 0.003）和血清阳性/未感染（P = 0.002）的中位CD 4 IFN-γ对EB的反应显著增加。在血清阳性/未感染的参与者中检测到对EB、PmpF和MOMP的阳性IFN-γ应答者的比率高于血清阴性/未感染的参与者（P = 0.021）。IL-17应答通常较低。IFN-γ对任何抗原的阳性反应与再感染风险降低的趋势相关，尽管没有统计学意义。在该青少年队列中，在急性感染和既往感染的参与者中检测到衣原体特异性CD 4 IFN-γ应答，但未检测到IL-17应答，阳性CD 4 IFN-γ应答与再感染风险无显著降低相关。(C)由Elsevier爱尔兰Ltd.出版
An evaluation of CD4 T cell responses to candidate Chlamydia trachomatis vaccine antigens was conducted in an adolescent female cohort exposed through natural infection to explore antigen immunogenicity and correlation with protection from reinfection. The frequency of peripheral blood CD4 T cell IFN-gamma and IL-17 responses to three candidate vaccine antigens, polymorphic membrane protein G (PmpG), F (PmpF), and major outer membrane protein (MOMP), were determined by ELISPOT; responses to chlamydial heat shock protein 60 (HSP60) and to elementary bodies (EB) were included for comparison. Responses of Infected (n = 8), Seropositive/Uninfected (n = 13), and Seronegative/Uninfected (n = 18) participants were compared. The median CD4 IFN-gamma response to EB was significantly increased in Infected (P = 0.003) and Seropositive/Uninfected (P = 0.002) versus Seronegative/Uninfected female subjects. Higher rates of positive IFN-gamma responders to EB, PmpF, and MOMP were detected in Seropositive/Uninfected versus Seronegative/Uninfected participants (P = 0.021). IL-17 responses were generally low. A positive IFN-gamma response to any of the antigens tested was associated with a trend toward a reduced risk of reinfection, although not statistically significant. Among this adolescent cohort, chlamydial-specific CD4 IFN-gamma but not IL-17 responses were detected in acutely and previously infected participants and a positive CD4 IFN-gamma response was associated with a non-significant reduced risk of reinfection. (C) 2014 Published by Elsevier Ireland Ltd.