Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.
Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.
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DOI：
10.1007/s00216-017-0406-7
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发表时间：
2017-07
影响因子：
4.3
通讯作者：
Azadi P
中科院分区：
文献类型：
--
作者：
Shajahan A;Heiss C;Ishihara M;Azadi P
糖蛋白的结构分析是一项具有挑战性的奋进，并且需求稳步增长，但只有非常有限的实验室拥有完成这项任务所需的专业知识。本教程针对分子生物学和生物化学领域的研究人员，他们发现糖蛋白在他们的生物学研究中很重要，并且正在寻找工具来阐明其结构。它提供了糖组学和糖蛋白组学分析中使用的主要和最常见的分析技术的简要描述，包括对各个步骤的原理的解释和对包含进行分析所需的实验细节的已发表文献的参考。糖组学包括对给定细胞或生物体中表达的聚糖的结构和功能的综合研究沿着对编码糖蛋白和糖基转移酶的所有基因的鉴定。糖蛋白质组学是糖组学和蛋白质组学的分支，是对含有糖的蛋白质进行翻译后修饰的研究。本教程旨在为那些没有碳水化合物分析经验的人轻松进入糖组学和糖蛋白质组学领域。
The structural analysis of glycoproteins is a challenging endeavor and is under steadily increasing demand, but only a very limited number of labs have the expertise required to accomplish this task. This tutorial is aimed at researchers from the fields of molecular biology and biochemistry that have discovered that glycoproteins are important in their biological research and are looking for the tools to elucidate their structure. It provides brief descriptions of the major and most common analytical techniques used in glycomics and glycoproteomics analysis, including explanations of the rationales for individual steps and references to published literature containing the experimental details necessary to carry out the analyses. Glycomics includes the comprehensive study of the structure and function of the glycans expressed in a given cell or organism along with identification of all the genes that encode glycoproteins and glycosyltransferases. Glycoproteomics which is subset of both glycomics and proteomics is the identification and characterization of proteins bearing carbohydrates as posttranslational modification. This tutorial is designed to ease entry into the glycomics and glycoproteomics field for those without prior carbohydrate analysis experience.