Evaluation of the differentiation potential of WB-F344 rat liver epithelial stem-like cells in vivo. Differentiation to hepatocytes after transplantation into dipeptidylpeptidase-IV-deficient rat liver.
Evaluation of the differentiation potential of WB-F344 rat liver epithelial stem-like cells in vivo. Differentiation to hepatocytes after transplantation into dipeptidylpeptidase-IV-deficient rat liver.
复制标题
DOI：
--
复制
发表时间：
1997-08
期刊：
影响因子：
--
通讯作者：
William B. Coleman;K. McCullough;G. Esch;R. Faris;D. Hixson;Gary J. Smith;J. Grisham
中科院分区：
文献类型：
--
作者：
William B. Coleman;K. McCullough;G. Esch;R. Faris;D. Hixson;Gary J. Smith;J. Grisham
将培养的WB-F344大鼠肝内移植到缺乏胆小管酶二肽酶IV(DPP-IV)的成年同基因德国品系Fischer 344大鼠的肝脏中，培养的WB-F344大鼠肝上皮细胞(没有外源标记基因)整合到肝板上，并分化为在形态和功能上与成熟肝细胞难以区分的肝细胞样细胞。在该模型系统中，移植的WB-F344细胞的分化后代通过其胆小管DPP-IV酶活性的表达在DPP-IV阴性的宿主肝细胞中进行鉴定。DPP-IV阳性的肝细胞样细胞还表达其他肝细胞分化标志，包括白蛋白、转铁蛋白和α-1-抗胰蛋白酶，提示移植的WB-F344细胞的后代表达完整的肝细胞分化程序。这些结果补充了我们之前的研究，表明WB-F344细胞可以作为分化肝细胞的干样前体细胞，并加强了WB-F344大鼠肝上皮细胞代表正常成年大鼠肝脏中存在的肝干样肝细胞前体细胞的培养对应物的说法。
After intrahepatic transplantation into livers of adult syngeneic German-strain Fischer 344 rats that are deficient for the bile canalicular enzyme dipeptidyl peptidase IV (DPP-IV), cultured WB-F344 rat liver epithelial cells (without exogenous marker genes) integrate into hepatic plates and differentiate into hepatocyte-like cells that are morphologically and functionally indistinguishable from mature hepatocytes. In this model system, the differentiated progeny of transplanted WB-F344 cells are identified among the DPP-IV-negative host hepatocytes by their expression of bile canalicular DPP-IV enzyme activity. DPP-IV-positive hepatocyte-like cells also expressed other markers of hepatocytic differentiation, including albumin, transferrin, and alpha-1-antitrypsin, suggesting that the progeny of transplanted WB-F344 cells express a complete hepatocyte differentiation program. These results complement our previous studies indicating WB-F344 cells can serve as stem-like precursor cells for differentiated hepatocytes and strengthen the suggestion that WB-F344 rat liver epithelial cells represent the cultured counterpart of liver stem-like hepatocyte progenitor cells present in the normal adult rat liver.