Gene flow creates a mirage of cryptic species in a Southeast Asian spotted stream frog complex
Gene flow creates a mirage of cryptic species in a Southeast Asian spotted stream frog complex
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DOI：
10.1111/mec.15603
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发表时间：
2020-09-14
影响因子：
4.9
通讯作者：
Brown, Rafe M.
中科院分区：
文献类型：
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作者：
Chan, Kin O.;Hutter, Carl R.;Brown, Rafe M.
大多数新的神秘物种描述使用传统的树和距离为基础的物种划界方法（SDM），依赖于系统发育的安排和遗传分化的措施。然而，虽然许多因素，如人口结构和基因流是众所周知的混淆系统发育推断和物种划界，这些过程的影响并不经常评估。使用大量的外显子，内含子，和ultraconserved元素获得使用FrogCap序列捕获协议，我们比较了传统的SDM与更强大的基因组分析，评估人口结构和基因流，以表征物种边界的东南亚青蛙复杂（Pulchrana picturata）。我们的研究结果表明，基因流动和渐渗可以产生类似于不同物种的系统发育模式和分歧水平（线粒体DNA分歧高达10%）。杂交种群被推断为独立的（单细胞）分支，与相邻种群高度分化（7%-10%）和异常相似（
Most new cryptic species are described using conventional tree- and distance-based species delimitation methods (SDMs), which rely on phylogenetic arrangements and measures of genetic divergence. However, although numerous factors such as population structure and gene flow are known to confound phylogenetic inference and species delimitation, the influence of these processes is not frequently evaluated. Using large numbers of exons, introns, and ultraconserved elements obtained using the FrogCap sequence-capture protocol, we compared conventional SDMs with more robust genomic analyses that assess population structure and gene flow to characterize species boundaries in a Southeast Asian frog complex (Pulchrana picturata). Our results showed that gene flow and introgression can produce phylogenetic patterns and levels of divergence that resemble distinct species (up to 10% divergence in mitochondrial DNA). Hybrid populations were inferred as independent (singleton) clades that were highly divergent from adjacent populations (7%-10%) and unusually similar (