Strain differences in seizure-induced cell death following pilocarpine-induced status epilepticus.
Strain differences in seizure-induced cell death following pilocarpine-induced status epilepticus.
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DOI：
10.1016/j.nbd.2011.08.013
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发表时间：
2012-01
影响因子：
6.1
通讯作者：
Schauwecker, P. Elyse
中科院分区：
文献类型：
--
作者：
Schauwecker, P. Elyse
小鼠品系在它们对尿素诱导的兴奋性毒性细胞死亡的易感性方面彼此不同。以前，我们已经证明，成熟的近交系小鼠在癫痫持续状态的红藻氨酸盐模型癫痫发作的神经病理后果的易感性显着的遗传差异。目前，虽然敏感性的应变依赖性差异的细胞机制仍不清楚，这种变化的一些假设有遗传基础。然而，目前尚不清楚是否在红藻氨酸给药后观察到的对苯脲诱导的细胞死亡的敏感性的菌株差异在全身给予其他化学惊厥剂后观察到。在啮齿类动物中，拟胆碱惊厥药毛果芸香碱被广泛用于诱导癫痫持续状态（SE），随后是海马损伤和自发性复发性癫痫发作，类似于颞叶癫痫。这种模型最初是在大鼠中描述的，但越来越多地用于小鼠。我们的特点后，匹罗卡品诱导的癫痫持续状态（SE）在8个近交系小鼠集中在海马神经元病理。使用匹鲁卡品的斜升剂量方案，并监测行为4-5小时。虽然我们没有观察到任何显着的差异，在癫痫发作的潜伏期或持续时间毛果芸香碱之间的近交系，我们观察到一个显着的差异敏感性毛果芸香碱诱导SE的神经病理后果。在筛选毛果芸香碱诱导状态的8种遗传多样性小鼠品系中，BALB/cJ和BALB/cByJ是仅有的两种对苯脲诱导细胞死亡的神经病理学后果具有抗性的品系。对这些小鼠品系的进一步研究可能有助于研究毛果芸香碱诱导的癫痫持续状态的遗传影响。
Mouse strains differ from one another in their susceptibility to seizure-induced excitotoxic cell death. Previously, we have demonstrated that mature inbred strains of mice show remarkable genetic differences in susceptibility to the neuropathological consequences of seizures in the kainate model of status epilepticus. At present, while the cellular mechanisms underlying strain-dependent differences in susceptibility remain unclear, some of this variation is assumed to have a genetic basis. However, it remains unclear whether strain differences in susceptibility to seizure-induced cell death observed following kainate administration are observed following systemic administration of other chemoconvulsants. In rodents, the cholinomimetic convulsant pilocarpine is widely used to induce status epilepticus (SE), followed by hippocampal damage and spontaneous recurrent seizures, resembling temporal lobe epilepsy. This model has initially been described in rats, but is increasingly used in mice. We characterized neuronal pathologies after pilocarpine-induced status epilepticus (SE) in eight inbred strains of mice focusing on the hippocampus. A ramping-up dose protocol for pilocarpine was used and behavior was monitored for 4–5 hours. While we did not observe any significant differences in seizure latency or duration to pilocarpine among the inbred strains, we did observe a significant difference in susceptibility to the neuropathological consequences of pilocarpine-induced SE. Of the eight genetically diverse mouse strains screened for pilocarpine-induced status, BALB/cJ and BALB/cByJ were the only two strains that were resistant to the neuropathological consequences of seizure-induced cell death. Additional studies of these murine strains may be useful for investigating genetic influences on pilocarpine-induced status epilepticus.
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影响因子：
5.3
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