PHYSIOLOGICAL METHOD FOR QUANTITATIVE MEASUREMENT OF MICROBIAL BIOMASS IN SOILS
PHYSIOLOGICAL METHOD FOR QUANTITATIVE MEASUREMENT OF MICROBIAL BIOMASS IN SOILS
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DOI：
10.1016/0038-0717(78)90099-8
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发表时间：
1978-01-01
影响因子：
9.7
通讯作者：
DOMSCH, KH
中科院分区：
文献类型：
--
作者：
ANDERSON, JPE;DOMSCH, KH
本文介绍了一种快速、客观地测定土壤中活的、非静止的微生物生物量中碳含量的方法。该方法基于微生物种群对过量C和能量源修正的初始呼吸反应，使用Jenkinson技术的扩展版本进行定量。对50个土壤样品同时测定的结果表明，两种方法的相关性极显著（r = 0.96）。从该相关性可以推断，在22 ° C下，C，底物诱导的最大呼吸速率为1 ml CO2。h-1对应于约40 mg微生物生物质C。从纯培养研究中获得了支持这些结果的证据。调查的各种土壤类型收集农业以及森林网站，它们含有15-240毫克微生物C. cntdot。100 g干土-1.呼吸方法提供了土壤改良后1-3小时内生物量大小的可重复估计。它可以毫无困难地与选择性抑制方法结合起来，用于确定细菌和真菌对土壤代谢的贡献。
A method is described for the rapid and objective estimation of the amount of C in the living, non-resting microbial biomass of soils. The method, which is based on the initial respiratory response of microbial populations to amendment with an excess of a C and energy source, was quantified using an expanded version of Jenkinson''s technique. The simultaneous application of the 2 methods to 50 soil samples showed a highly significant correlation (r = 0.96) between both. From this correlation it could be deduced that at 22.degree. C, a substrate-induced maximal respiratory rate of 1 ml CO2 .cntdot. h-1 corresponds to about 40 mg microbial biomass C. Evidence supporting these results was obtained from pure culture studies. The various soil types investigated were collected from agricultural as well as forest sites and they contained 15-240 mg microbial C .cntdot. 100 g dry soil-1. The respiratory method provides reproducible estimates of biomass size within 1-3 h after soil amendment. It can be combined without difficulty with a selective inhibition method for determination of bacterial and fungal contributions to soil metabolism.