SEVERE AND PROGRESSIVE STEATOSIS AND FOCAL NECROSIS IN RAT-LIVER INDUCED BY CONTINUOUS INTRAGASTRIC INFUSION OF ETHANOL AND LOW FAT DIET
SEVERE AND PROGRESSIVE STEATOSIS AND FOCAL NECROSIS IN RAT-LIVER INDUCED BY CONTINUOUS INTRAGASTRIC INFUSION OF ETHANOL AND LOW FAT DIET
复制标题
DOI：
10.1002/hep.1840050212
复制
发表时间：
1985-01-01
期刊：
影响因子：
13.5
通讯作者：
LARGMAN, C
中科院分区：
文献类型：
--
作者：
TSUKAMOTO, H;FRENCH, SW;LARGMAN, C
血液酒精水平（BAL）维持在高水平（14只酗酒大鼠在216.0 +-）。雄性Wistar大鼠通过连续灌胃乙醇和营养定义的低脂流质饮食，持续15-85天。为了保持高BAL，乙醇摄入量从总热量的32%逐渐增加到41.4%。配对喂养的动物分别接受等热量葡萄糖溶液和液体饲料。尽管饮食脂肪含量很低（占总热量的4.9%），但肝脏的组织病理学评估显示，酒精大鼠的脂肪浸润严重且呈进行性。此外，中毒30天后，1/3的动物肝脏小叶中心区出现局灶性坏死，单核细胞浸润。这与这些动物血清谷草转氨酶（SGOT）和血清谷水转氨酶（SGPT）水平显著升高有关。配对喂养的对照组肝脏形态和血液化学均未见异常。肝脏甘油三酯的化学定量证实了组织学观察，甘油三酯水平为61.51 .+-。16.45和89.61 .+-。第30天和第85天分别为5.94 mg/g。脂肪变性程度与平均BAL达到紧密且显著相关（r = 0.80, P < 0.001）。持续高BAL与酒精性肝脏病理的严重程度相关。
Blood alcohol levels (BAL) were maintained at high levels (in 14 alcoholic rats at 216.0 .+-. 120.1 mg%) in male Wistar rats for 15-85 days by continuous intragastric infusion of ethanol and nutritionally defined low fat liquid diet. The ethanol intake was progressively increased from 32% of total calories up to 41.4% in order to maintain high BAL. Pair-fed animals received isocaloric glucose solution and the liquid diet. Despite the low level of dietary fat (4.9% of total calories), histopathological evaluation of the liver revealed severe and progressive fatty infiltration in the alcoholic rats. In addition, following 30 days of intoxication, 1/3 of the animals showed focal necrosis with mononuclear cell infiltration in centrilobular areas of the livers. This was correlated with the markedly elevated levels of SGOT [serum glutamic oxaloacetic transaminase] and SGPT [serum glutamic syruvic transaminase] in these animals. Pair-fed controls showed no abnormality in the morphology of liver or blood chemistry. Chemical quantitation of liver triglycerides confirmed the histological observations, with triglyceride levels of 61.51 .+-. 16.45 and 89.61 .+-. 5.94 mg/g at 30 and 85 days, respectively. The degree of steatosis was tightly and significantly correlated with the mean BAL achieved (r = 0.80, P < 0.001). Continuously high BAL correlate with the severity of alcohol-induced liver pathology.