Rigidity of silicone substrates controls cell spreading and stem cell differentiation.
Rigidity of silicone substrates controls cell spreading and stem cell differentiation.
复制标题
有机硅基底的刚性控制细胞扩散和干细胞分化。
DOI：
10.1038/srep33411
复制
发表时间：
2016
影响因子：
4.6
通讯作者：
Tkachenko,Eugene
中科院分区：
文献类型：
--
作者：
Vertelov,Grigory;Gutierrez,Edgar;Lee,Sin-Ae;Ronan,Edward;Groisman,Alex;Tkachenko,Eugene
铺在水凝胶和硅凝胶基质上的干细胞的扩散和分化对基质的刚性和孔隙率的依赖性最近一直是一些争议的主题。在将人间充质干细胞接种在软、中等硬度和硬硅胶上的实验中，我们发现硬凝胶更具有成骨性，软凝胶更具有成脂性，并且细胞铺展面积随着硅胶基质硬度的增加而增加。我们的研究结果表明，基底刚性会诱导一些普遍的细胞反应，而与基底的孔隙率或地形无关。
The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show that harder gels are more osteogenic, softer gels are more adipogenic, and cell spreading areas increase with the silicone gel substrate rigidity. The results of our study indicate that substrate rigidity induces some universal cellular responses independently of the porosity or topography of the substrate.