OsIAA23-mediated auxin signaling defines postembryonic maintenance of QC in rice
OsIAA23-mediated auxin signaling defines postembryonic maintenance of QC in rice
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DOI：
10.1111/j.1365-313x.2011.04698.x
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发表时间：
2011-11-01
期刊：
影响因子：
7.2
通讯作者：
Wu Ping
中科院分区：
文献类型：
--
作者：
Ni Jun;Wang Gaohang;Wu Ping
虽然静止中心（QC）是至关重要的根发育，调控其胚后维持的分子机制仍然不清楚。在这项研究中，一个半显性突变体，表现出多效性缺陷的根组织，其中包括根冠，侧根和冠根，被隔离。该突变体的特征在于在胚后发育期间QC身份的丧失，并且所展示的缺陷是由OsIAA 23（Os 06 g39590）的结构域II中的稳定突变引起的。0 sIAA 23的表达特异于初生根、侧根和冠根发育过程中根尖的QC。与0 sIAA 23在QC中的表达一致，由DR 5 p：：GUS（β-葡萄糖醛酸酶）标记的生长素信号传导在0 sIAA 23的QC区域中不存在。在QHB启动子控制下含有Osiaa 23的转基因水稻植物部分模拟Osiaa 23的缺陷。这些结果表明QC的维持依赖于QC中OsIAA 23介导的生长素信号传导。这些研究结果提供了深入了解Aux/IAA为基础的生长素信号在胚后维护的QC植物。
Although the quiescent center (QC) is crucial to root development, the molecular mechanisms that regulate its postembryonic maintenance remain obscure. In this study, a semi-dominant mutant that exhibits pleiotropic defects in root tissues, which includes the root cap, lateral and crown roots, was isolated. The mutant is characterized by a loss of QC identity during postembryonic development, and the displayed defects result from a stabilizing mutation in domain II of OsIAA23 (Os06g39590). Expression of OsIAA23 is specific to the QC of the root tip during the development of primary, lateral and crown roots. Consistent with OsIAA23 expression in the QC, the auxin signaling marked by DR5p::GUS (beta-glucuronidase) was absent in the QC region of Osiaa23. Transgenic rice plants harboring Osiaa23 under the control of the QHB promoter mimic partially the defects of Osiaa23. These results indicate that the maintenance of the QC is dependent on OsIAA23-mediated auxin signaling in the QC. These findings provide insight into Aux/IAA-based auxin signaling during postembryonic maintenance of the QC in plants.