A NOVEL PROGRAMMED FRAMESHIFT EXPRESSES THE POL3 GENE OF RETROTRANSPOSON-TY3 OF YEAST - FRAMESHIFTING WITHOUT TRANSFER-RNA SLIPPAGE
A NOVEL PROGRAMMED FRAMESHIFT EXPRESSES THE POL3 GENE OF RETROTRANSPOSON-TY3 OF YEAST - FRAMESHIFTING WITHOUT TRANSFER-RNA SLIPPAGE
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DOI：
10.1016/0092-8674(93)90297-4
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发表时间：
1993-07-16
期刊：
影响因子：
64.5
通讯作者：
VIMALADITHAN, A
中科院分区：
文献类型：
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作者：
FARABAUGH, PJ;ZHAO, H;VIMALADITHAN, A
大多数逆转录病毒和反转录转座子将Pol1基因表达为与上游Gag基因的翻译融合，通常涉及翻译移码。我们在这里描述了发生在酵母反转录转座子Ty3的GAG3和POL3基因之间的一种不寻常的翻译移码事件。在GCG AGU U(显示为GAG3的密码子)序列中发生+1移码，编码丙氨酸-缬氨酸(GCG A GUU)。与所描述的其他程序化翻译框架转移不同，这一事件不需要mRNA中同源或近同源密码子之间的tRNA滑动。GCG密码子远端的两个特征会刺激移码。针对‘’饥饿‘’丝氨酸密码子AGU的tRNA的低可获得性导致了移码所需的翻译暂停。AGU密码子末端12个核苷酸的序列(称为Ty3‘’上下文‘’)也刺激该事件。
Most retroviruses and retrotransposons express their pol gene as a translational fusion to the upstream gag gene, often involving translational frameshifting. We describe here an unusual translational frameshift event occurring between the GAG3 and POL3 genes of the retrotransposon Ty3 of yeast. A + 1 frameshift occurs within the sequence GCG AGU U (shown as codons of GAG3), encoding alanine-valine (GCG A GUU). Unlike other programed translational frameshifts described, this event does not require tRNA slippage between cognate or near-cognate codons in the mRNA. Two features distal to the GCG codon stimulate frameshifting. The low availability of the tRNA specific for the ''hungry'' serine codon, AGU, induces a translational pause required for frameshifting. A sequence of 12 nt distal to the AGU codon (termed the Ty3 ''context'') also stimulates the event.