The similarity of chorionic gonadotrophin and its subunits in term placentae from man, apes, old and New World monkeys and a prosimian.
The similarity of chorionic gonadotrophin and its subunits in term placentae from man, apes, old and New World monkeys and a prosimian.
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人、猿、新世界猴和原猴足月胎盘中绒毛膜促性腺激素及其亚基的相似性。
DOI：
10.1159/000155965
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
L. Wide
中科院分区：
文献类型：
--
作者：
B. Hobson;L. Wide
用生物测定法和放射免疫测定法测定了11种猿和猴胎盘提取物中绒毛膜促性腺激素（CG）的含量。两种测定系统的结果之间存在显著相关性（r = 0.903，p <0.001）。大多数灵长类动物足月胎盘中CG的浓度与人类足月胎盘中的浓度相同。所有胎盘提取物与羊LH-β亚基的抗血清发生交叉反应，黑猩猩和大猩猩的提取物与HCG-β亚基的羧基末端肽的抗血清也发生了显著的交叉反应。在Sephadex G-200上层析的灵长类胎盘具有在HCG、HCG-α HCG-β亚基的RIA系统中具有活性的组分。一般而言，所有猿和猴胎盘提取物的洗脱曲线类似于从人类足月胎盘和纯化的HCG及其亚基制备的洗脱曲线。来自人和非人材料的洗脱模式的形状表明存在不止一种分子形式的CG-α亚基活性。第二种，具有β亚基活性的更延迟的分子形式在由人、大猩猩、长颈鹿和恒河猴胎盘制成的提取物中发现。猿、猴胎盘CG结构与HCG及其亚单位的相似性提示其在妊娠后期具有与HCG相似的功能。
Chorionic gonadotrophin (CG) was estimated, by bioassay and radioimmunoassay (RIA), in placental extracts from 11 ape and monkey species. There was a significant correlation between the results of the two assay systems (r = 0.903, p less than 0.001). The concentration of CG in most primate term placentae was the same as that in the human placenta at term. Extracts from all placentae cross-reacted with antiserum to ovine LH-beta subunit, and those of the chimpanzee and gorilla also had a significant cross-reaction with an antiserum to the carboxyl terminal peptide of the HCG-beta subunit. Primate placentae chromatographed on Sephadex G-200 had components active in the RIA systems for HCG, HCG-alpha HCG-beta subunits. In general, the elution profiles of all ape and monkey placental extracts resemble those made from human term placentae and of purified HCG and its subunits. The shape of the elution patterns from human and non-human material suggests that there was more than one molecular form of CG-alpha subunit activity. A second, more retarded molecular form having beta subunit activity was found in extracts made from human, gorilla, gibbon and rhesus monkey placentae. The similarity between the structure of ape and monkey placental CG with HCG and its subunits implies a function similar to that of HCG in late pregnancy.