Significant increase of self-renewal in hematopoietic cells after forced expression of EVI1
Significant increase of self-renewal in hematopoietic cells after forced expression of EVI1
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DOI：
10.1016/j.bcmd.2007.07.012
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发表时间：
2008-03-01
影响因子：
2.3
通讯作者：
Nucifora, Giuseppina
中科院分区：
文献类型：
--
作者：
Laricchia-Robbio, Leopoldo;Nucifora, Giuseppina
EVI1首次被鉴定为MDS1/EVI1基因组位点上的嗜生性逆转录病毒的优先整合位点，导致易感小鼠的髓系肿瘤。后来的研究表明，MDS1/EVI1基因座上的逆转录病毒整合导致在不敏感的小鼠品系、非人类灵长类动物和患者中出现了非恶性的显性造血干细胞克隆，这表明该基因座编码的基因可能会影响细胞的自我更新潜力。该基因座编码两个基因。其中一种是EVI1，长期以来一直与髓系白血病有关。为了了解EVI1是否在自我更新控制中起作用，我们在两个增殖和自我更新潜力不同的小鼠的骨髓祖细胞中强制表达了EVI1。通过比较EVI1对造血细胞的反应，我们得出结论：EVI1具有延长细胞自我更新潜能的作用，并且EVI1的这种能力受到细胞固有特性的限制和调节。(C)2007 Elsevier Inc.保留所有权利。
EVI1 was first identified as a preferential integration site of ecotropic retroviruses in the MDS1/EVI1 genomic locus leading to myeloid tumors in susceptible mice. Later studies showed that retroviral integration in the MDS1/EVI1 locus results in the emergence of a non-malignant dominant hematopoietic stem cell clone in non-susceptible mice strains, in non-human primates, and in patients, suggesting that a gene encoded by the locus could affect the self-renewal potential of a cell. The locus encodes two genes. One of them, EVI1, has long been associated with myeloid leukemia. To understand whether EVI1 has a role in self-renewal control, we forcibly expressed EVI1 in the bone marrow progenitors of two mice strains that differ in their proliferation and self-renewal potential. By comparing the response of the hematopoietic cells to EVI1, we conclude that EVI1 has a role in prolonging the self-renewal potential of the cells and that this ability of EVI1 is limited and modulated by inherent characteristics of the cells. (c) 2007 Elsevier Inc. All rights reserved.