Differential effects of selenite and selenate on human melanocytes, keratinocytes, and melanoma cells
Differential effects of selenite and selenate on human melanocytes, keratinocytes, and melanoma cells
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DOI：
10.1139/o04-130
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发表时间：
2005-04-01
期刊：
影响因子：
--
通讯作者：
Korohoda, W
中科院分区：
文献类型：
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作者：
Bandura, L;Drukala, J;Korohoda, W
近年来引起肿瘤学家注意的物质中有含硒化合物，既有无机的也有有机的。一些流行病学研究表明，硒摄入量与癌症发病率呈负相关。在本文报道的实验中，我们比较了两种不同氧化水平的无机含硒盐，亚硒酸盐IV和亚硒酸盐VI的影响。我们测试了这两种化合物对正常角质形成细胞、黑素细胞和人黑色素瘤细胞系HTB140三种类型的人皮肤细胞的细胞存活和生长、细胞周期处理、细胞形态、细胞骨架和脂质过氧化的影响。报道了亚硒酸盐和亚硒酸盐对正常细胞和肿瘤细胞的活力、生长和形态的不同影响，为今后研究和治疗某些肿瘤疾病提供了基础。重点研究了亚硒酸盐诱导的细胞凋亡，并推测了受试物质对硫氧还蛋白还原酶系统的影响。
Among the substances that attracted the attention of oncologists in recent years are selenium-containing compounds, both inorganic and organic. Several epidemiological studies have shown an inverse correlation between selenium intake and cancer incidence. In the experiments reported here, we compared the effects of 2 inorganic selenium- containing salts that differed in the level of selenium oxidation, selenite IV and selenate VI. We tested the effects of these 2 compounds on cell survival and growth, cell cycle processing, cell morphology, cytoskeleton, and lipid peroxidation in 3 human skin cell types: normal keratinocytes, melanocytes, and human melanoma cell line HTB140. The different effects of selenite and selenate on the viability, growth, and morphology of normal cells and tumor cells are reported and provide a base for future research and treatment of some neoplastic diseases. The attention is paid to cell apoptosis induced by selenite and not by selenate, and the effects of tested substances on thioredoxin reductase system are postulated.