Natural Killer Cell Receptor+ T-Lymphocytes in Normal and Helicobacter pylori-Infected Human Gastric Mucosa
Natural Killer Cell Receptor+ T-Lymphocytes in Normal and Helicobacter pylori-Infected Human Gastric Mucosa
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DOI：
10.1111/j.1523-5378.2008.00641.x
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发表时间：
2008-12-01
期刊：
影响因子：
4.4
通讯作者：
Moran, Anthony P.
中科院分区：
文献类型：
--
作者：
O'Keeffe, Joan;Gately, Carol M.;Moran, Anthony P.
幽门螺杆菌感染与慢性炎症的发展和免疫细胞浸润到胃粘膜中有关。由于表达自然杀伤细胞受体的非常规T淋巴细胞被认为在抗感染的免疫应答中起核心作用，一项研究调查了它们在正常和H.采用流式细胞术对从胃粘膜上皮和固有层新鲜分离的淋巴细胞中表达自然杀伤细胞标志物CD 161、CD 56和CD 94的T细胞进行定量。十三H pylori阳性和24例H. CD94（+）T细胞是H. pylori的上皮和固有层中最丰富的（高达40%）自然杀伤受体阳性T细胞群。幽门阴性胃粘膜。CD161（+）T细胞约占两个隔室中所有T细胞的三分之一，但比例最低的是CD56（+）T细胞。与H. pylori阴性粘膜，H.在幽门螺杆菌感染的粘膜中，上皮中的CD161（+）T细胞数量显著增加（p = 0.04），而固有层中的CD56（+）T细胞数量较低（p = 0.01）。在H的两个粘膜层中存在少量T细胞（< 2%）。pylori阴性的受试者是自然杀伤T细胞，其比例与H.自然杀伤细胞受体阳性T细胞在胃粘膜内不同位置的优势、异质性和分布反映了在H. pylori感染，需要进一步研究。
Helicobacter pylori infection is associated with development of chronic inflammation and infiltration of immune cells into the gastric mucosa. As unconventional T-lymphocytes expressing natural killer cell receptors are considered to play central roles in the immune response against infection, a study investigating their frequencies in normal and H. pylori-infected gastric mucosa was undertaken.Flow cytometry was used to quantify T-cells expressing the natural killer cell markers CD161, CD56, and CD94 in freshly isolated lymphocytes from the epithelial and lamina propria layers of gastric mucosa. Thirteen H. pylori-positive and 24 H. pylori-negative individuals were studied.CD94(+) T-cells were the most abundant (up to 40%) natural killer receptor-positive T-cell population in epithelial and lamina propria layers of H. pylori-negative gastric mucosa. CD161(+) T-cells accounted for about one-third of all T-cells in both compartments, but the lowest proportion were of CD56(+) T-cells. Compared with H. pylori-negative mucosa, in H. pylori-infected mucosa the numbers of CD161(+) T-cells were significantly greater (p = .04) in the epithelium, whereas the numbers of CD56(+) T-cells were lower (p = .01) in the lamina propria. A minor population (< 2%) of T-cells in both mucosal layers of H. pylori-negative subjects were natural killer T-cells, and whose proportions were not significantly different (p > .05) to those in H. pylori-infected individuals.The predominance, heterogeneity, and distribution of natural killer cell receptor-positive T-cells at different locations within the gastric mucosa reflects a potential functional role during H. pylori infection and warrants further investigation.