Genetical variation for enzyme activity in a population of Drosophila melanogaster. vi. molecular variation in the control of alcohol dehydrogenase (ADH) activity
Genetical variation for enzyme activity in a population of Drosophila melanogaster. vi. molecular variation in the control of alcohol dehydrogenase (ADH) activity
复制标题
果蝇群体中酶活性的遗传变异。
作者：
A. Birley;P. Couch;A. Marson
对16个高自交系的成年雌性和雄性黑腹果蝇72小时龄的ADH活性、25°时的ADH活性、免疫测定的ADH蛋白水平和体重进行了测定。ADHF等位基因纯合的2个品系的ADH活性和ADH蛋白水平最高。在14个ADHS/S品系中，ADH蛋白水平的变异与ADH活性的遗传变异有关([RCirc]=0.6)。在16个自交系中，ADH活性或ADH蛋白水平与体重或总蛋白之间的遗传相关性均不显著。从两个ADH活性较高和较低的自交系中筛选出8个代表I、II和III染色体全部纯合组合的自交系，对它们的ADH活性、ADH蛋白和总蛋白进行了研究。结果表明，ADH活性受到由染色体III控制的翻译后事件的显著改变，总蛋白受不同于控制ADH活性的染色体效应的影响。两个ADHF/F和上述两个ADHS/S品系的蝇类粗提物对异丙醇的亲和力有明显差异。
SummaryFour characters, ADH activity at 25°, immunologically determined ADH protein level, total protein and body weight were measured upon 72 hour old adult female and male Drosophila melanogaster from 16 highly inbred lines, derived from the laboratory population, “Texas” (established 1966). The highest levels of ADH activity and ADH protein level were observed in the 2 lines homozygous for the AdhF allele. Amongst the 14 AdhS/S lines variation for ADH protein level was associated with genetical variation for ADH activity ([rcirc ] = 0·6). The genetical association between ADH activity or ADH protein level and either body weight or total protein in the 16 inbred lines was not statistically significant. A study of ADH activity, ADH protein and total protein in 8 lines representing all homozygous combinations of chromosomes I, II and III and derived from two inbred AdhS/S lines, chosen for their respective high and low ADH activities, showed that ADH activity was considerably modified by a post-translational event controlled from chromosome III. Total protein was controlled by different chromosomal effects from those controlling ADH activity. Michaelis constants for crude fly extracts of the two AdhF/F and the above two AdhS/S lines showed clear differences in affinity for isopropanol.