MicroRNA-22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3
MicroRNA-22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3
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MicroRNA-22 通过靶向 NLRP3 抑制口腔鳞状细胞癌的细胞增殖、迁移和侵袭。
DOI：
10.1002/jcp.26331
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发表时间：
2018-09-01
影响因子：
5.6
通讯作者：
Chen, Fuxiang
中科院分区：
文献类型：
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作者：
Feng, Xiaodong;Luo, Qingqiong;Chen, Fuxiang
MicroRNAs （miRNAs）已被认为是致癌和肿瘤发展的重要调节因子。最近，有报道称microRNA-22 （miR-22）是几种类型肿瘤中与癌症相关的miRNA。在这项研究中，我们旨在探讨miR-22在口腔鳞状细胞癌（OSCC）中的作用。我们发现，与癌旁非癌组织相比，miR-22在OSCC组织中的表达明显降低。此外，慢病毒介导的miR-22过表达显著降低了OSCC细胞的活力、迁移和侵袭，而miR-22抑制剂促进了这些参数。在机制上，NLR家族pyrin结构域包含三个（NLRP3）被确定为miR-22的直接靶点。在OSCC组织和细胞系中，miR-22的表达与NLRP3的表达呈负相关。此外，在OSCC细胞中过表达miR-22可以逆转激活的NLRP3炎性体的促肿瘤作用，反之亦然。因此，我们的研究结果表明，miR-22可能通过靶向NLRP3在OSCC中发挥抑制作用，这为OSCC生长和转移的分子机制提供了新的见解。
MicroRNAs (miRNAs) have been implicated as important regulators of carcinogenesis and tumor development. Recently, microRNA-22 (miR-22) has been reported to be a cancer-related miRNA in several types of tumors. In this study, we aimed to investigate the role of miR-22 in oral squamous cell carcinoma (OSCC). We found that miR-22 expression was significantly decreased in OSCC tissues compared with that in the adjacent noncancerous tissues. Furthermore, lentivirus-mediated miR-22 overexpression markedly reduced OSCC cell viability, migration and invasion, whereas miR-22 inhibitor promoted these parameters. Mechanistically, NLR family pyrin domain containing three (NLRP3) was identified as a direct target of miR-22. miR-22 expression was inversely correlated with NLRP3 expression both in OSCC tissues and cell lines. Moreover, overexpression of miR-22 in OSCC cells could reverse the tumor-promoting effect of the activated NLRP3 inflammasome and vice versus. Therefore, our results indicate that miR-22 may play a suppressive role in OSCC by targeting NLRP3, which offer new insights into the molecular mechanisms of the growth and metastasis of OSCC.