Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae.
Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae.
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DOI：
10.1186/1471-2164-10-461
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发表时间：
2009-10-05
期刊：
影响因子：
4.4
通讯作者：
Penttilä M
中科院分区：
文献类型：
--
作者：
Rintala E;Toivari M;Pitkänen JP;Wiebe MG;Ruohonen L;Penttilä M
工业上重要的酵母酿酒酵母在有氧和无氧条件下都能生长。然而，在中间氧可用性条件下对其代谢的调节并没有很好地表征。我们评估了在厌氧和好氧条件下，以及在三种中间氧水平（0.5、1.0和2.8%）的原料气中，氧气供应对葡萄糖限制的酿酒酵母的转录组和蛋白质组的影响。转录组的主要差异是在完全好氧、中间氧和厌氧条件下的比较中观察到的，而在不同中间氧水平（0.5、1.0或2.8% O2）的饲料气条件下转录组基本不变。转录组和蛋白质组数据的比较表明，转录后调控是重要的，特别是在0.5%氧条件下。在中氧条件下，编码呼吸途径酶的基因比在有氧或无氧条件下表达得更高。类似的趋势也出现在蛋白质组和TCA循环的酶活性中。此外，与完全有氧条件相比，编码线粒体翻译机制蛋白质的基因在所有缺氧和无氧条件下都存在更高的水平。在中间氧条件下，编码呼吸通路组分的基因的整体上调表明，作为对更有效的能量生产需求的响应，存在一种控制这些基因的调节机制。此外，在三种不同的中间氧水平下生长的细胞在转录水平上高度相似，而在蛋白质组水平上却存在差异，这表明转录后机制导致了不同的呼吸发酵代谢生理模式。
The industrially important yeast Saccharomyces cerevisiae is able to grow both in the presence and absence of oxygen. However, the regulation of its metabolism in conditions of intermediate oxygen availability is not well characterised. We assessed the effect of oxygen provision on the transcriptome and proteome of S. cerevisiae in glucose-limited chemostat cultivations in anaerobic and aerobic conditions, and with three intermediate (0.5, 1.0 and 2.8% oxygen) levels of oxygen in the feed gas. The main differences in the transcriptome were observed in the comparison of fully aerobic, intermediate oxygen and anaerobic conditions, while the transcriptome was generally unchanged in conditions receiving different intermediate levels (0.5, 1.0 or 2.8% O2) of oxygen in the feed gas. Comparison of the transcriptome and proteome data suggested post-transcriptional regulation was important, especially in 0.5% oxygen. In the conditions of intermediate oxygen, the genes encoding enzymes of the respiratory pathway were more highly expressed than in either aerobic or anaerobic conditions. A similar trend was also seen in the proteome and in enzyme activities of the TCA cycle. Further, genes encoding proteins of the mitochondrial translation machinery were present at higher levels in all oxygen-limited and anaerobic conditions, compared to fully aerobic conditions. Global upregulation of genes encoding components of the respiratory pathway under conditions of intermediate oxygen suggested a regulatory mechanism to control these genes as a response to the need of more efficient energy production. Further, cells grown in three different intermediate oxygen levels were highly similar at the level of transcription, while they differed at the proteome level, suggesting post-transcriptional mechanisms leading to distinct physiological modes of respiro-fermentative metabolism.
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影响因子：
--
作者：
Buschlen, S;Amillet, JM;Bolotin-Fukuhara, M
通讯作者：
Bolotin-Fukuhara, M
影响因子：
3.4
作者：
Almeida, JS;Stanislaus, R;Arthur, JM
通讯作者：
Arthur, JM
影响因子：
4.8
作者：
Burke, PV;Raitt, DC;Poyton, RO
通讯作者：
Poyton, RO
影响因子：
14.9
作者：
Cohen, BD;Sertil, O;Lowry, CV
通讯作者：
Lowry, CV
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y