Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.
Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.
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DOI：
10.4161/rna.29946
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发表时间：
2014
期刊：
影响因子：
4.1
通讯作者：
Niessing D
中科院分区：
文献类型：
--
作者：
Niedner A;Edelmann FT;Niessing D
不对称的、依赖运动蛋白的mrna转运和随后的本地化翻译是基因调控的重要机制。由于这种运动粒子的高度复杂性，我们对mRNA定位的机制理解有限。在过去的二十年里，ASH1 mRNA在出芽酵母中的定位已经成为一个相对简单和可接近的模型系统。最近的研究进展帮助我们对ASH1 mRNA从共转录产生到到达目的地的分子机制有了越来越清晰的认识。这些新的见解有助于更好地理解初始核mrna的需求，通过顺式作用RNA元件特异性mrna -货物识别的分子基础，RNP生物发生和重组的不同阶段，以及货物结合时运动活性的激活。我们在其他模式生物发表的研究结果的背景下讨论这些方面。
Asymmetric, motor-protein dependent transport of mRNAs and subsequent localized translation is an important mechanism of gene regulation. Due to the high complexity of such motile particles, our mechanistic understanding of mRNA localization is limited. Over the last two decades, ASH1 mRNA localization in budding yeast has served as comparably simple and accessible model system. Recent advances have helped to draw an increasingly clear picture on the molecular mechanisms governing ASH1 mRNA localization from its co-transcriptional birth to its delivery at the site of destination. These new insights help to better understand the requirement of initial nuclear mRNPs, the molecular basis of specific mRNA-cargo recognition via cis-acting RNA elements, the different stages of RNP biogenesis and reorganization, as well as activation of the motile activity upon cargo binding. We discuss these aspects in context of published findings from other model organisms.