Growth of human stem cell-derived neurons on solid three-dimensional polymers
Growth of human stem cell-derived neurons on solid three-dimensional polymers
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DOI：
10.1016/j.jbbm.2004.12.001
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发表时间：
2005-03-31
影响因子：
--
通讯作者：
Przyborski, SA
中科院分区：
文献类型：
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作者：
Hayman, MW;Smith, KH;Przyborski, SA
了解神经分化和三维基质中复杂神经突网络的发育是体外神经组织工程的关键。在这项研究中，我们首次描述了人胚胎干细胞衍生的神经元在涂有生物活性分子的固体聚苯乙烯基质上的生长。以高内相乳液（HIPE）为模板，以苯乙烯/二乙烯基苯共聚物为原料制备了多孔泡沫材料。所得的聚HIPE基质容易用包括聚-D-赖氨酸和层粘连蛋白的水基溶液包被。人类神经元粘附以及聚-D-赖氨酸涂层的表面和延长的神经过程，然而，神经突生长特别增强时，聚合物也收到了涂层的层粘连蛋白。这些数据清楚地证明了固体聚苯乙烯支架在创造细胞生长和分化的三维环境中的潜在用途。我们建议，这些强大的和稳定的矩阵可以方便地和常规地用于组织培养实验室中，以研究在三维生长的细胞的行为。(c)2004 Elsevier B. V.保留所有权利。
Understanding neural differentiation and the development of complex neurite networks in three-dimensional matrices is critical for neural tissue engineering in vitro. In this study we describe for the first time the growth of human stein cell-derived neurons on solid polystyrene matrices coated with bioactive molecules. Highly porous foams were prepared from poly(styrene/divinylbenzene) using a high internal phase emulsion (HIPE) as a template to create the porous structure. The resulting polyHIPE matrices were readily coated with aqueous-based solutions including poly-D-lysine and laminin. Human neurons adhered well to poly-D-lysine coated surfaces and extended neural processes, however, neurite outgrowth was particularly enhanced when polymers also received a coating of laminin. These data clearly demonstrate the potential use of solid polystyrene scaffolds to create three-dimensional environments for cell growth and differentiation. We propose that these robust and stable matrices can be conveniently and routinely used in the tissue culture laboratory to study the behaviour of cells grown in three-dimensions. (c) 2004 Elsevier B.V. All rights reserved.