The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue.
The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue.
复制标题
雌激素对大鼠脂肪组织脂蛋白脂肪酶活性的影响。
DOI：
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发表时间：
1975
影响因子：
15.9
通讯作者：
P. Hamosh
中科院分区：
文献类型：
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作者：
M. Hamosh;P. Hamosh
在雄性和切除卵巢的雌性大鼠中研究了17 β-雌二醇或孕酮给药对脂肪组织脂蛋白脂酶活性的影响。脂蛋白脂肪酶活性测定丙酮-乙醚提取的脂肪组织制剂与双标记（14 C-脂肪酸，3 H-甘油）乳糜微粒甘油三酯作为底物。雄性大鼠给予17 β-雌二醇后，脂肪组织脂蛋白脂酶活性从8.22 ± 1.8 U/g（1 U = 1 mumol甘油三酯水解/h）降至4.96 ± 0.5 U/g。卵巢切除使脂肪组织脂蛋白脂酶活性从对照组的10.4 ± 1.8 U/g增加到22.7 ± 4.3 U/g。对卵巢切除大鼠给予17 β-雌二醇可导致脂肪组织脂蛋白脂酶活性显著下降：17 β-雌二醇（2.5 μ g/天）将酶活性降至9.00 ± 1.2 U/g，而25 μ g/天进一步将脂蛋白脂酶活性降至3.2 ± 0.6 U/g。血液甘油三酯水平从卵巢切除大鼠的0.8 ± 0.05 mumol/ml增加到25 mug/天17 β-雌二醇治疗大鼠的1.4 ± 0.09 mumol/ml。在雄性或卵巢切除大鼠中，给予Progestrin并不影响脂肪组织脂蛋白脂酶活性。心脏和肺脂蛋白脂酶活性不受激素治疗的影响。我们认为，血液甘油三酯浓度的上升，伴随着高雌激素水平，可能是由于脂肪组织脂蛋白脂酶活性的显着抑制。
The effect of 17beta-estradiol or progesterone administration on adipose tissue lipoprotein lipase activity was studied in male and ovariectomized female rats. Lipoprotein lipase activity was measured in acetone-ether-extracted preparations of adipose tissue with doubly labeled (14C-fatty acid, 3H-glyceryl) chylomicron triglyceride as substrate. Administration of 17beta-estradiol to male rats lowered adipose tissue lipoprotein lipase activity from 8.22 plus or minus 1.8 U/g (1 U = 1 mumol triglyceride hydrolyzed per h) to 4.96 plus or minus 0.5 U/g in the treated group. Ovariectomy increased adipose tissue lipoprotein lipase activity from 10.4 plus or minus 1.8 U/g in controls to 22.7 plus or minus 4.3 U/g. 17beta-Estradiol administration to ovariectomized rats cuased a marked fall in adipose tissue lipoprotein lipase activity: 17beta-estradiol (2.5 mug/day) lowered the enzyme activity to 9.00 plus or minus 1.2 U/g, whereas 25 mug/day further decreased lipoprotein lipase activity to 3.2 plus or minus 0.6 U/g. Blood triglyceride levels increased from 0.8 plus or minus 0.05 mumol/ml in ovariectomized rats to 1.4 plus or minus 0.09 mumol/ml in 25 mug/day 17beta-estradiol-treated rats. Progesterone administration did not affect adipose tissue lipoprotein lipase activity in either male or ovariectomized rats. Heart and lung lipoprotein lipase activity was unaffected by hormone treatment. We suggest that the rise in blood triglyceride concentrations, which accompanies high palsma estrogen levels, could be due to the marked inhibition of adipose tissue lipoprotein lipase activity.