Gas-Phase Ion-Ion Reactions for Lipid Identification in Biological Tissue Sections.
Gas-Phase Ion-Ion Reactions for Lipid Identification in Biological Tissue Sections.
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DOI：
10.1007/978-1-0716-2030-4_1
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发表时间：
2022
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明确识别同量异位（即相同的标称质量）和异构（即相同的精确质量）脂质仍然是成像质谱 (IMS) 工作流程中具有挑战性但至关重要的方面。本章介绍了生物组织样品的制备方法以及使用混合质谱仪进行气相电荷反转离子/离子反应以改进脂质鉴定的方法。这种气相离子/离子反应方法提供的脂质结构信息超出了通过传统串联质谱 (MS/MS) 实验所能获得的信息。虽然此处描述的此程序用于使用 1,4-亚苯基二丙酸试剂二价阴离子来鉴定磷脂酰胆碱 (PC) 分析物阳离子，但它可以很容易地推广到执行各种离子/离子反应化学。
The unambiguous identification of isobaric (i.e., same nominal mass) and isomeric (i.e., same exact mass) lipids remains a challenging yet vital aspect of imaging mass spectrometry (IMS) workflows. This chapter presents a methodology for the preparation of biological tissue samples and the use of a hybrid mass spectrometer to perform gas-phase charge inversion ion/ion reactions for improved lipid identification. This gas-phase ion/ion reaction method provides lipid structural information beyond what can be obtained via conventional tandem mass spectrometry (MS/MS) experiments. While this procedure is described here for the identification of phosphatidylcholine (PC) analyte cations using 1,4-phenylenedipropionic acid reagent dianions, it can readily be generalized to perform a diverse array of ion/ion reaction chemistries.