A non-invasive system to measure heart rate in hard-shelled sea turtles: potential for field applications
A non-invasive system to measure heart rate in hard-shelled sea turtles: potential for field applications
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DOI：
10.1098/rstb.2020.0222
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发表时间：
2021-08-02
影响因子：
6.3
通讯作者：
Sato, Katsufumi
中科院分区：
文献类型：
--
作者：
Sakamoto, Kentaro Q.;Miyayama, Masaru;Sato, Katsufumi
为了测量无拘无束的海龟的心率，人们认为，由于贝壳的存在，必须在体内插入一个探头。然而，插入探头是侵入性的，很难应用于野外动物。在这里，我们开发了一种对某些种类的海龟进行无创心率测量的方法。在我们的方法中，使用动物携带的心电记录器和两个电极--它们与海水绝缘--粘贴在甲壳上，进行心电检查。根据测量的心电信号，采用带通滤波的算法对心跳信号进行识别。我们在一个用户友好的程序包ECGtoHR中实现了该算法。在水箱和泻湖中进行的实验中，我们成功地测量了黑头海龟、橄榄海龟和黑海龟的心率，但没有测量到绿海龟和鹰嘴龟的心率。海龟在水下休息时的平均心率为6.2+/-1.9次/分(-1)，在水面活动时的平均心率为14.0+/-2.4次/分(-1)。我们的方法特别适用于濒临灭绝的物种，如海龟，并有可能扩展到其他各种自由放养物种。这篇文章是主题问题“测量自由生活的动物的生理学(第一部分)”的一部分。
To measure the heart rate of unrestrained sea turtles, it has been believed that a probe must be inserted inside the body owing to the presence of the shell. However, inserting the probe is invasive and difficult to apply to animals in the field. Here, we have developed a non-invasive heart rate measurement method for some species of sea turtles. In our approach, an electrocardiogram (ECG) was performed using an animal-borne ECG recorder and two electrodes-which were electrically insulated from seawater-pasted on the carapace. Based on the measured ECG, the heartbeat signals were identified with an algorithm using a band-pass filter. We implemented this algorithm in a user-friendly program package, ECGtoHR. In experiments conducted in a water tank and in a lagoon, we successfully measured the heart rate of loggerhead, olive ridley and black turtles, but not green and hawksbill turtles. The average heart rate of turtles when resting underwater was 6.2 +/- 1.9 beats min(-1) and that when moving at the surface was 14.0 +/- 2.4 beats min(-1). Our approach is particularly suitable for endangered species such as sea turtles, and has the potential to be extended to a variety of other free-ranging species.This article is part of the theme issue 'Measuring physiology in free-living animals (Part I)'.