Characterization of Tissue Response to Impact Loads Delivered Using a Hand-Held Instrument for Studying Articular Cartilage Injury
Characterization of Tissue Response to Impact Loads Delivered Using a Hand-Held Instrument for Studying Articular Cartilage Injury
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DOI：
10.1177/1947603515595071
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发表时间：
2015-10-01
期刊：
影响因子：
2.8
通讯作者：
Bonassar, Lawrence J.
中科院分区：
文献类型：
--
作者：
Bonnevie, Edward D.;Delco, Michelle L.;Bonassar, Lawrence J.
目的：本研究的目的是充分表征体内打入器的力学特性，并将力学与关节面的表面开裂相关联。设计：使用弹簧加载的撞击器对新生牛软骨关节面施加能量控制的撞击。同时使用的负荷传感器和位移传感器提供的应力，应力率，应变，应变率，应变能密度的测量。在冲击后应用印度墨水用于将冲击期间的机械输入与所导致的组织损伤的严重程度相关联。此外，开发并验证了从冲击应力中反卷积惯性应力的信号处理方法。结果：影响模型与数据拟合良好（均方根误差平均值接近0.09），并提供了充分表征的影响。通过印度油墨应用可见的机械输入和表面开裂程度之间的相关性分析提供了应力和应力速率与表面开裂程度的显着正相关性（R-2 = 0.7398和R-2 = 0.5262，分别）。冲击参数的范围为7至21 MPa，6至40 GPa/s，0.16至0.38，87至236 s（-1），和0.3至1.1 MJ/m（3）的应力，应力速率，应变，应变速率和应变能密度分别。在13 MPa、15 GPa/s、0.23、160 s（-1）和0.59 MJ/m（3）条件下测定了该系统所有输入的损伤阈值。结论：本研究为使用便携式、可灭菌和可拆卸的撞击器械提供了力学基础。使用该装置能够在体外或体内控制冲击载荷，将机制研究与疾病进展的长期监测联系起来。
Objective: The objective of this study was to fully characterize the mechanics of an in vivo impactor and correlate the mechanics with superficial cracking of articular surfaces. Design: A spring-loaded impactor was used to apply energy-controlled impacts to the articular surfaces of neonatal bovine cartilage. The simultaneous use of a load cell and displacement sensor provided measurements of stress, stress rate, strain, strain rate, and strain energy density. Application of India ink after impact was used to correlate the mechanical inputs during impact with the resulting severity of tissue damage. Additionally, a signal processing method to deconvolve inertial stresses from impact stresses was developed and validated. Results: Impact models fit the data well (root mean square error average similar to 0.09) and provided a fully characterized impact. Correlation analysis between mechanical inputs and degree of superficial cracking made visible through India ink application provided significant positive correlations for stress and stress rate with degree of surface cracking (R-2 = 0.7398 and R-2 = 0.5262, respectively). Ranges of impact parameters were 7 to 21 MPa, 6 to 40 GPa/s, 0.16 to 0.38, 87 to 236 s(-1), and 0.3 to 1.1 MJ/m(3) for stress, stress rate, strain, strain rate, and strain energy density, respectively. Thresholds for damage for all inputs were determined at 13 MPa, 15 GPa/s, 0.23, 160 s(-1), and 0.59 MJ/m(3) for this system. Conclusions: This study provided the mechanical basis for use of a portable, sterilizable, and maneuverable impacting device. Use of this device enables controlled impact loads in vitro or in vivo to connect mechanistic studies with long-term monitoring of disease progression.