2-SITE SANDWICH ENZYME-IMMUNOASSAY WITH MONOCLONAL-ANTIBODIES TO HUMAN ALPHA-FETOPROTEIN
2-SITE SANDWICH ENZYME-IMMUNOASSAY WITH MONOCLONAL-ANTIBODIES TO HUMAN ALPHA-FETOPROTEIN
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DOI：
10.1016/0022-1759(81)90219-2
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发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
ENGVALL, E
中科院分区：
文献类型：
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作者：
UOTILA, M;RUOSLAHTI, E;ENGVALL, E
使用与甲胎蛋白(AFP)分子上的不同抗原决定簇反应的单克隆抗体来开发简化的夹心酶联免疫特异性测定，其中固定1个单克隆抗体，而另一个用酶标记。由于其不同的特异性，附着在固相上的抗体和缀合有酶的抗体不会竞争与 AFP 的结合，从而可以在一次短暂的孵育中完成所需的 2 个抗原-抗体反应。该测定的快速性、简单性和出色的灵敏度，加上与使用 2 个单克隆抗体相关的精致特异性，表明基于此原理的测定将可用于对具有医学和生物学重要性的物质进行定量。
Monoclonal antibodies reacting with different antigenic determinants on the .alpha.-fetoprotein (AFP) molecule were used to develop a simplified sandwich enzyme-linked immunospecific assay in which 1 monoclonal antibody was immobilized and another labeled with an enzyme. Due to their different specificities the antibody attached to the solid phase and the one conjugated to the enzyme did not compete for binding to AFP making it possible to complete the 2 antigen-antibody reactions required in a single, short incubation. The rapidity, simplicity and excellent sensitivity of this assay combined with the exquisite specificity associated with the use of 2 monoclonal antibodies indicate that assays based on this principle will be useful in the quantitation of substances of medical and biological importance.