Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels
Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels
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DOI：
10.1007/s004180100328
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发表时间：
2001-10-01
影响因子：
2.3
通讯作者：
Kreczy, A
中科院分区：
文献类型：
--
作者：
Debbage, PL;Sölder, E;Kreczy, A
我们之前在小鼠模型中应用活体凝集素灌注来阐明血管通透性的机制。目前的工作将这种技术转移到人体模型，分析宏观和微血管的血管通透性。人血管内皮表面碳水化合物的生物化学与小鼠血管内皮表面碳水化合物的生物化学明显不同，缺乏α -半乳糖表位，在糖萼中表达L-聚焦部分；聚n -乳胺聚糖骨架是所有哺乳动物共有的。我们在切片和体内广泛检测了凝集素的结合特异性，然后将聚n -乳糖胺特异性凝集素LEA和L-聚焦特异性凝集素UEA-I应用于人体活体灌注。大血管和微血管的内皮转运不同。在成人脂肪组织、直肠壁和直肠癌的微血管中，囊泡经内皮转运缓慢，随后在内皮下基底膜有明显滞留；未观察到细胞旁传代。通过时间超过1小时。因此，我们发现了类似于我们之前在小鼠组织中描述的屏障机制。在成人和胎儿大血管中，分别观察到隐大静脉和脐静脉快速穿过内皮内膜，示踪剂在15分钟内完全定位于内皮下组织；小泡运输比微血管更快，在内皮下基底膜的滞留时间更短。
We previously applied intravital lectin perfusion in mouse models to elucidate mechanisms underlying vascular permeability. The present work transfers this technique to human models, analysing vascular permeability in macro- and microvessels. Human vascular endothelial surface carbohydrate biochemistry differs significantly from its murine counterpart, lacking alpha -galactosyl epitopes and expressing the L-fucose moiety in the glycocalyx; the poly-N-lactosamine glycan backbone is common to all mammals. We examined extensively lectin binding specificities in sections and in vivo, and then applied the poly-N-lactosamine-specific lectin LEA and the L-fucose-specific lectin UEA-I in human intravital perfusions. Transendothelial transport differed in macrovessels and microvessels. In microvessels of adult human fat tissue, rectal wall and rectal carcinomas, slow transendothelial transport by vesicles was followed by significant retention at the subendothelial basement membrane; paracellular passage was not observed. Passage time exceeded 1 h. Thus we found barrier mechanisms resembling those we described previously in murine tissues. In both adult and fetal macrovessels, the vena saphena magna and the umbilical vein, respectively, rapid passage across the endothelial lining was observed, the tracer localising completely in the subendothelial tissues within 15 min; vesicular transport was more rapid than in microvessels, and retention at the subendothelial basement membrane briefer.