An automatic method for determination of urinary 3-methylhistidine: normal values.
An automatic method for determination of urinary 3-methylhistidine: normal values.
复制标题
尿 3-甲基组氨酸的自动测定方法：正常值。
DOI：
10.1016/0003-2697(79)90662-6
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发表时间：
1979
影响因子：
2.9
通讯作者：
P. Fürst
中科院分区：
文献类型：
--
作者：
M. Neuhäuser;P. Fürst
本文介绍了一种尿中3-甲基组氨酸的自动分析系统，该系统采用离子交换色谱法，采用自动进样器、电动选择阀和二极管编程器控制分析系统。该方法允许22个样品/天的采样率。3-甲基组氨酸在37分钟内从组氨酸中完全分离，而1-甲基组氨酸与氨一起洗脱。3-甲基组氨酸浓度在高达150 nmol/ml时呈线性，在自动连续运行中未发现明显的样品相互作用。在基于重复样品的单次测定中，误差为4.61%，在重复测定中，误差为3.26%。12名健康女性和12名健康男性的平均尿3-甲基组氨酸排出量分别为299.4 ± 23.8 μmol/d和545.5 ± 35.2 μmol/d。当表示为绝对日排出量或与体重、体表面积或肌酐的估计比值时，雄性的3-甲基组氨酸排泄量显著高于雌性。
A system for automatic analysis of urinary 3-methylhistidine is described, applying ion-exchange chromatography and using an automatic sample injector, a motoric selector valve, and a diode programmer, which controls the analytical system. The method permits a sampling rate of 22 samples/day. 3-Methylhistidine was completely separated from histidine in 37 min whereas 1-methylhistidine was eluted together with ammonia. The 3-methylhistidine concentration was linear up to 150 nmol/ml and no appreciable sample interaction was found at automatic sequential runs. The error, in a single determination based on duplicate samples, was 4.61% and, in duplicated determinations, 3.26%. The mean urinary 3-methylhistidine output was 299.4 ± 23.8 μmol/day in 12 healthy females and 545.5 ± 35.2 μmol/day in 12 healthy males. The 3-methylhistidine excretion was significantly higher in males than in females, when expressed as the absolute daily output or as the estimated ratio to body weight, body surface area, or creatinine.