Isolation of crustacean hyperglycemic hormone-family peptides with vitellogenesis-inhibiting activity from the shiba shrimp Metapenaeus joyneri.
Isolation of crustacean hyperglycemic hormone-family peptides with vitellogenesis-inhibiting activity from the shiba shrimp Metapenaeus joyneri.
复制标题
从柴虾乔内新对虾中分离具有卵黄形成抑制活性的甲壳动物高血糖激素家族肽。
DOI：
10.1007/s12562-014-0834-7
复制
发表时间：
2015
影响因子：
1.9
通讯作者：
Tsuyoshi Ohira
中科院分区：
文献类型：
--
作者：
Midori Fukushima;Sayaka Kotaka;Naoaki Tsutsui;Kiyoshi Asahina;Susumu Izumi;Tsuyoshi Ohira
甲壳动物卵黄发生抑制激素(VIH)属于甲壳类高血糖激素(CHH)家族。为了研究中国对虾Metapenaeus joyneri中的多种VIH分子，用反相高效液相色谱分离纯化了4个CHH家族多肽Mej-SGP-I、-II、-III和-IV，并对其N端氨基酸序列进行了鉴定。用日本对虾卵巢碎片体外孵育的方法检测了这些多肽对卵黄发生的抑制作用。这四种多肽都显著降低了卵巢碎片中卵黄蛋白原的mRNA水平。其中，鼻窦腺中含量最丰富的CHH家族多肽Mej-SGP-III具有明显的抑制活性。因此，利用RT-PCR结合5‘-和3’-RACE的方法，克隆了编码MeJ-SGP-III前体的基因。成熟的MeJ-SGP-III由72个氨基酸残基组成，含有6个保守的半胱氨酸残基，并具有一个酰胺化的C末端。成熟的Mej-SGP-III与Pej-SGP-V(六种CHH/VIHs INM之一)具有最高的序列同源性。JAPONICUS)和Liv-SGP-G(凡纳滨对虾六种VIHs之一)。这些结果表明，Mej-SGP-III是调节卵黄发生的激素之一。乔纳利。
Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. In order to characterize multiple VIH molecules in the shiba shrimpMetapenaeus joyneri, four CHH-family peptides designated as Mej-SGP-I, -II, -III, and -IV were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The vitellogenesis-inhibiting activities of these peptides were examined using ex vivo incubation of ovarian fragments of the kuruma prawnMarsupenaeus japonicus. All of the four peptides significantly reduced vitellogenin mRNA levels in the ovarian fragments. Among them, the inhibitory activity of Mej-SGP-III which was the most abundant CHH-family peptide in the sinus gland was apparent. Therefore, a cDNA encoding the Mej-SGP-III precursor was cloned by RT-PCR coupled with 5′- and 3′-RACE. The mature Mej-SGP-III was found to consist of 72 amino acid residues containing six conserved cysteine residues and possess an amidated C-terminus. The mature Mej-SGP-III showed the highest sequence identity to Pej-SGP-V (one of six CHH/VIHs inM. japonicus) and Liv-SGP-G (one of six VIHs inLitopenaeus vannamei). These results indicate that Mej-SGP-III is one of the hormones regulating vitellogenesis inM. joyneri.