INHIBITORY EFFECT OF CHLOROPHYLL ON THE GENOTOXICITY OF 3-AMINO-1-METHYL-5H-PYRIDO[4,3-B]INDOLE (TRP-P-2)
INHIBITORY EFFECT OF CHLOROPHYLL ON THE GENOTOXICITY OF 3-AMINO-1-METHYL-5H-PYRIDO[4,3-B]INDOLE (TRP-P-2)
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DOI：
10.1093/carcin/10.1.145
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发表时间：
1989-01-01
期刊：
影响因子：
4.7
通讯作者：
HAYATSU, H
中科院分区：
文献类型：
--
作者：
NEGISHI, T;ARIMOTO, S;HAYATSU, H
叶绿素可抑制3-氨基-1-甲基-5H-吡啶并[4，3-B]吲哚（Trp-P-2）对果蝇的遗传毒性。使用翅毛斑试验，我们发现，形成的突变体的头发在成年苍蝇作为一个结果，喂养他们与色氨酸-2在其幼虫阶段被有效地抑制共同管理叶绿素。斑点频率的降低取决于叶绿素的剂量，并且在使用的最高剂量下，其中Trp-P-2与叶绿素的重量比为1：80，观察到完全防止小的单斑点形成。叶绿素的发色团叶绿酸也有类似的抑制作用。在研究抑制机制的研究中，我们观察到3-羟基-氨基-1-甲基-5H-吡啶并[4，3-B]吲哚[Trp-P-2-（NHOH）]（Trp-P-2的代谢活化形式）在鼠伤寒沙门氏菌TA 98中的致突变性被叶绿素和叶绿酸有效地抑制。我们还发现叶绿素和叶绿素可以与Trp-P-2和Trp-P-2（NHOH）形成复合物。这些抑制的直接机制是Trp-P-2 [和Trp-P-2（NHOH）]在形成叶绿素复合物时不再可用于生物体。
Genotoxicity of 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult flies as a result of feeding them with Trp-2 in their larval stage was efficiently inhibited by coadministration on chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxy-amino-1-methyl-5H-pyrido[4,3-b]indole [Trp-P-2-(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyll can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.