Abnormal expression of molecular markers for bladder impermeability and differentiation in the urothelium of patients with interstitial cystitis
Abnormal expression of molecular markers for bladder impermeability and differentiation in the urothelium of patients with interstitial cystitis
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DOI：
10.1097/01.ju.0000118938.09119.a5
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发表时间：
2004-04-01
影响因子：
6.6
通讯作者：
Culkin, DJ
中科院分区：
文献类型：
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作者：
Slobodov, G;Feloney, M;Culkin, DJ
目的：尽管间质性膀胱炎（IC）的病因学缺乏共识，但尿路上皮的不通透性丧失和其他异常是该疾病的特征。在本研究中，通过 E-钙粘蛋白、ZO-1、尿斑蛋白和硫酸软骨素的免疫组织化学评估，探讨了尿路上皮活检中与上皮粘附、细胞分化和膀胱不通透性相关的蛋白质分布。 材料和方法：将从 27 名 IC 患者和 7 名对照患者获得的活检组织立即固定在福尔马林中，对所述蛋白质进行免疫组织化学标记，并对蛋白质表达、形态和功能进行评分。结果：只有 3 个 IC 样本完全正常，而 27 个样本中的 24 个至少 I 标记物显示异常，6 个样本中所有 4 个标记物均异常。在患者与对照组中，尿斑蛋白异常，27 例中有 13 例，7 例中有 1 例（p=0.085）；E-钙粘蛋白异常（过表达），27 例中有 18 例，7 例中有 0 例（p=0.0021）；ZO-1 异常，27 例中有 11 例，7 例中有 0 例（p=0.046）；硫酸软骨素异常，27 例中有 15 例，与对照组相比，硫酸软骨素异常。 7 中的 0 (p=0.0054)。形态/极性评分与 ZO-1（Pearson r=0.3935，p=0.0423）和硫酸软骨素（Pearson r=0.7079，p）显着相关
Purpose: Despite a lack of consensus concerning the etiology of interstitial cystitis (IC) the loss of impermeability and other abnormalities of the urothelium are features of the disease. In this study the distribution of proteins involved with epithelial adhesion, cellular differentiation and bladder impermeability in urothelial biopsies were explored by the immunohistochemical assessment of E-cadherin, ZO-1, uroplakin and chondroitin sulfate.Materials and Methods: Biopsies obtained from 27 patients with IC and 7 controls were immediately fixed in formalin, immunohistochemically labeled for the described proteins and scored for protein expression, morphology and differentiation.Results: Only 3 IC samples appeared completely normal, while 24 of the 27 showed an abnormality in at least I marker and in 6 all 4 markers were abnormal. In patients vs controls findings were abnormal for uroplakin in 13 of 27 vs 1 of 7 (p=0.085), for E-cadherin (over expressed) in 18 of 27 vs 0 of 7 (p=0.0021), for ZO-1 in 11 of 27 vs 0 of 7 (p=0.046) and for chondroitin sulfate in 15 of 27 vs 0 of 7 (p=0.0054). The morphology/polarity score significantly correlated with ZO-1(Pearson r=0.3935, p=0.0423) and chondroitin sulfate (Pearson r=0.7079, p