Senescent Fibroblast-Derived GDF15 Induces Skin Pigmentation
Senescent Fibroblast-Derived GDF15 Induces Skin Pigmentation
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DOI：
10.1016/j.jid.2020.04.016
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发表时间：
2020-12-01
影响因子：
6.5
通讯作者：
Kang, Hee Young
中科院分区：
文献类型：
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作者：
Kim, Yeongeun;Kang, Bogyeong;Kang, Hee Young
衰老的成纤维细胞在衰老的色素沉着中起作用。在这项研究中，我们发现GDF15在紫外线照射的衰老成纤维细胞和光老化的色素沉着的皮肤中的表达水平增加。为了研究GDF15对黑素合成的影响，将正常人黑素细胞与感染GDF15慢病毒或GDF15短发夹状RNA的成纤维细胞共同培养。研究发现，GDF15通过β-连环蛋白信号途径上调MITF/酪氨酸酶，从而刺激黑素细胞黑素生成。在体外培养的皮肤和重组人皮肤样本中，进一步证实了GDF15在色素沉着过程中的刺激作用。这些结果表明，衰老成纤维细胞来源的GDF15刺激皮肤色素沉着，并可能在衰老相关的色素沉着中发挥作用。
Senescent fibroblasts play a role in aging pigmentation. In this study, we found that GDF15 expression levels are increased in UV-irradiated senescent fibroblasts and photoaged hyperpigmented skin. To investigate the effects of GDF15 on melanogenesis, normal human melanocytes were cocultured with fibroblasts infected with the GDF15 lentivirus or GDF15 short hairpin RNA. It was found that GDF15 stimulates melanogenesis in melanocytes through MITF/tyrosinase upregulation via beta-catenin signaling. The stimulatory action of GDF15 during pigmentation was further confirmed in ex vivo cultured skin and in a reconstituted human skin sample. These results suggest that senescent fibroblast-derived GDF15 stimulates skin pigmentation and may play a role in aging-associated pigmentation.