Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain
Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain
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DOI：
10.1007/s00253-006-0402-x
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发表时间：
2006-10-01
影响因子：
5
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Katahira, Satoshi;Mizuike, Atsuko;Kondo, Akihiko
森工木屑等木质纤维生物质的硫酸水解物是生产燃料生物乙醇的重要原料。本研究通过整合树干毕赤酵母木糖还原酶和木糖醇脱氢酶基因、酿酒酵母木糖激酶基因和Acleatus曲霉β-葡萄糖苷酶基因，构建了一株能发酵木糖和低聚纤维低聚糖的重组酵母菌株。在木屑硫酸水解液的发酵中，36h后木糖和低聚纤维低聚糖发酵完全，开始时添加总糖73g/L生产30g/L左右的乙醇。在此情况下，该重组酵母的乙醇产量明显高于对照酵母。这些结果表明，具有木糖同化和纤维低聚糖降解能力的重组酵母菌能够有效地发酵木质纤维素水解物。
The sulfuric acid hydrolysate of lignocellulosic biomass, such as wood chips, from the forest industry is an important material for fuel bioethanol production. In this study, we constructed a recombinant yeast strain that can ferment xylose and cellooligosaccharides by integrating genes for the intercellular expressions of xylose reductase and xylitol dehydrogenase from Pichia stipitis, and xylulokinase from Saccharomyces cerevisiae and a gene for displaying beta-glucosidase from Aspergillus acleatus on the cell surface. In the fermentation of the sulfuric acid hydrolysate of wood chips, xylose and cellooligosaccharides were completely fermented after 36 h by the recombinant strain, and then about 30 g/l ethanol was produced from 73 g/l total sugar added at the beginning. In this case, the ethanol yield of this recombinant yeast was much higher than that of the control yeast. These results demonstrate that the fermentation of the lignocellulose hydrolysate is performed efficiently by the recombinant Saccharomyces strain with abilities for xylose assimilation and cellooligosaccharide degradation.