cDNA cloning of the immunoglobulin heavy chain genes in banded houndshark Triakis scyllium
cDNA cloning of the immunoglobulin heavy chain genes in banded houndshark Triakis scyllium
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DOI：
10.1016/j.fsi.2010.07.034
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发表时间：
2010-11-01
影响因子：
4.7
通讯作者：
Aoki, Takashi
中科院分区：
文献类型：
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作者：
Honda, Yuka;Kondo, Hidehiro;Aoki, Takashi
在本研究中，从斑点叉尾鲨(Triakis Scyllium)中克隆了免疫球蛋白(Ig)重链分泌型IgM、IgNAR和IGW的cDNA。Ig M重链的两个克隆编码569和570个氨基酸，其保守的(C)区与其他软骨鱼的氨基酸同源性为47-70%。其中4个克隆编码673-670个氨基酸，具有保守的Ig超家族结构域。IgNAR C区的氨基酸序列与已报道的氨基酸序列有56-69%的同源性。高通量测序表明，在大多数IgNAR序列中，两个可变区(CDR1和CDR3)各含有一个半胱氨酸残基。鉴定出三种类型的IGW，一种含有其他软骨鱼类中的Ig超家族结构域，一种在恒定区中缺少第三结构域，另一种缺乏第三到第五结构域。尽管有这些差异，但IGW亚型与其他软骨鱼的IgW聚在一起，C区显示出47%-%的氨基酸同源性。IgM和IgNAR的mRNAs在不同组织中均有表达，而IGW的mRNAs主要在胰腺中表达。带状猎鲨也有IgM、IGW和IgNAR，以及其他具有独特IGW亚型的软骨鱼。(C)2010爱思唯尔有限公司。保留所有权利。
In this study, cDNAs encoding the secreted forms of the immunoglobulin (Ig) heavy chains of IgM, IgNAR, and IgW were cloned from the banded houndshark Triakis scyllium. Two clones for the IgM heavy chains encoded 569 and 570 amino acids, whose conserved (C) region showed 47-70% amino acid identities to those reported in other cartilaginous fish. Four clones for the IgNAR encoded 673-670 amino acids with conserved Ig-superfamily domains. The IgNAR C region showed 56-69% amino acid identities to those so far reported. High-throughput sequencing revealed that in most of the IgNAR sequences, the two variable regions (CDR1 and CDR3) each possess a cysteine residue. Three types of IgW were identified; one contained Ig-superfamily domains that are in other cartilaginous fish, one lacks the 3rd domain in the constant region, and one lacks the 3rd to 5th domains. Despite these differences, the IgW isoforms clustered with IgWs of other cartilaginous fishes and the C regions showed 47-89% amino acid identities. mRNAs for IgM and IgNAR were detected in various tissues, while IgW mRNA was mainly detected in pancreas. The banded hounded shark also has IgM, IgW and IgNAR as well as the other cartilaginous fish with unique IgW isoform. (C) 2010 Elsevier Ltd. All rights reserved.